Persistence and Survival of Dried Sperm DNA under Ambient Conditions by Lay-Hong Seah in JFSCI - Juniper Publishers
The survival of sperm DNA in semen stains could be attributed by the resilient, protective structure of the sperm head which enables DNA in the sperm nucleus to survive for prolonged periods of time. The separation profiles of sperm DNA mixtures by differential extraction are peculiar only to sperm cells and thought to be associated with the intrinsic properties of semen. Experiments with dried semen on fabric stored under ambient conditions tested over a period of nine months (realistic maximum time for forensic casework submission) demonstrated the persistence and survival of sperm DNA with no allelic or locus dropouts. The separation profiles of sperm mixtures in the aging experiments detected no variations in the separation patterns of major-minor mixtures underlining the reliability of dried aged semen stains for interpretation in forensic casework.
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