Friday, January 29, 2021

Advancing Electronic Waste Management Techniques among Electrical/Electronic Technicians’ Workshops for Sustainable Healthy Society - Juniper Publishers

 Mining Science & Technology - Juniper Publishers


Abstract

This study focused on the advancement of e-waste techniques among electrical/electronic technicians’ workshops for sustainable healthy society. Study was conducted in Nigeria. The participants for the study included 87 university engineering lecturers and 54 public health officers. Study adopted survey research design and structured questionnaire for data collection. The study was validated by three experts and reliability coefficient of 0.79 was achieved. Data obtained was analyzed using percentage, mean and standard deviation while t-test and ANOVA were used to test hypotheses. The result claimed that all e-waste components are hazardous except aluminum. Meanwhile, the level of hazardous varies and depends on type of e-waste and level of abundance. Result claimed that e-waste in electrical/electronic technician workshop have severe consequences such as uncontrolled fire and inflammatory/respiratory problem among others in the environment. Result confirmed that electrical/electronic technicians adopted unsafe method for managing e-waste such as dumping of e-waste inside flowing water and swamp and using e-waste for land filling. Result explained further that electrical/electronic technicians are facing challenges- exposure to injury and unavailability of modern equipment among others- in managing e-waste. Also, result confirmed that technique for managing e-waste in electrical/electronic workshop include establishment of recycling site and establishment and implementation of regulation. Meanwhile, result stated that qualification, experience, age and occupation affects respondent opinions in response to research questions.

Keywords: Electrical/Electronic Technicians, Electrical/Electronic Workshops, E-Waste, Hazardous Component of E-Waste Material, E-Waste Management

Introduction

Educators and sociologists often conceived technicians as junior professional workers who know some proper subset of what professionals such as engineers or scientists know [1-3]. Technicians are workers in the field of technology who are proficient in relevant skills and techniques, with relatively practical understanding of theoretical principles for the purpose of carrying out installation, maintenance and repair activities [4,5]. Engineering technicians are charged with responsibilities like testing, monitoring, identifying, correcting and repairing (troubleshooting) problem that may arise on devices [6,7]. Generally, the ultimate function, discipline and way of life of technicians is to troubleshoot and ensure that machines and other physical systems remain in good working order [8]. Meanwhile, during the 1950s and 1960s, technician was routinely cross-referenced with “radio and television” as well as “electronics” repair [9]. This marks the beginning of electrical/electronic technicians.

Electrical/Electronic Technicians

Seigler [10] opined that electrical/electronic technician is a person working on electrical/electronic equipment at technical level between the skilled tradesman and the professional electrical engineer/scientists who has the technical knowledge and skill necessary to repair and service the modern-day consumer electronic products. In Nigeria, electrical/electronic technicians are trained in technical colleges to acquire necessary skills and competencies to carryout maintenance and repair activities such as dismantling, assembling, repairing, servicing, maintaining and installing electrical/electronic equipment and home appliances [11-14]. Arthur, et al. [1]; Seigler [10]; Dearden [15]; Bureau of Labour Statistics [16], Onuoha [17] reported that aside from upholding the responsibility of training apprentices, electrical/electronic technicians are charged to advice consumers, plan, design, develop, construct, assemble, erect, install, maintain, repair, adjust, monitor, service, test and commission electrical equipment/appliances. Seigler [10] asserted that most of electrical/electronic technicians carry out their function and maintenance activities in service shops or in stores popularly termed as workshop - a place where technicians and learners/ apprentices experiment, test, construct, dismantle, repair, design, create, imagine and study [18-20]. Electrical/Electronic technicians troubleshoot, maintain and repair appliances and equipment of different types such as refrigerator and air-conditions, electric motors and generators, voltage regulators, electric fan, Radio and Television sets, Amplifiers and Video recorders among others [11,13]. To be candid, the work of electrical/electronic technicians are acknowledged, recognized and supported globally. Experts expatiated that manufacturers of electrical/ electronic appliances are providing supportive skill training to technicians which enable technicians of electrical/electronic appliances to carry out repairing and maintenance activities to take faulty equipment back to their normal working condition for economic concern [21-24].

Meanwhile, ageing, shorter life span, emergence of new and latest technology, obsoleteness of existing technology, scarcity of spare parts to rectify faulty equipment, poor technological knowhow behind electrical/electronic product among the technicians, failure to afford charging/billing price or service requirement by consumers among other factors cause some electrical/electronic products and appliances to be accumulated at technician workshops or stores [25-30]. Onuoha [17] opined that most of electrical/electronic workshops are the house of junks for unrepaired and obsolete equipment. Also, Amachree [31]; Puckett, Westervelt, Gutierrez and Takamiya [32] explained that one quarter of the fairly used electrical/electronic appliances imported to Nigeria are functional, while the remaining three quarter of these appliances are either electronic junk or unserviceable at electrical/electronic workshop. Babatunde [25] argued that these junks of electrical/electronic equipment constitute wastes of Electrical and Electronic Equipment (Waste- EEE), which can otherwise be called Electronic Waste (E-Waste) or e-scraps at technicians’ workshops. Olaitan, Asogwa and Abu [23] reported that electrical/electronic machines and appliances become e-waste when they are considered out of use for their predetermined purposes.

E-waste

Kozlan [33] opined that e-waste is all electrical/electronic equipment or products with power plug, and batteries which have become obsolete due to advancement in technology, changes in fashion, style and status, and nearing the end of their useful life. Morgan [34] described e-waste as loosely discarded, surplus, obsolete or broken electrical or electronic devices. Electrical/ electronic waste refers to electrical/electronic appliances designed with a voltage rating not exceeding 1000 volts for alternating current and 1500 volts for direct current which have become obsolete, at the end of their lives or that have been discarded by their original users [35-38]. Babatunde [25]; Mundada, Sunil, & Shekdar, [26]; Ewuim, Akunne, Abajue, Nwankwo & Faniran [39]; Eyo [40]; Ogbomo, Obuh & Ibolo [41] opined that e-waste could be mobile phones, computers, communication equipment, entertainment electronic gadgets, household electronic appliances, audio-visual equipment and other valuable items or less functional and durable electronic gadgets that are no longer in use by their original owners. Scholars submitted that e-waste, in most of the time, is grouped into large household appliances, small household appliances, information technology (IT) and telecommunication equipment and consumer equipment [38,39,42,43].Emphatically, it is very undoubtful to declare that Massive volume and tons of e-waste are produced globally [44]. Specifically, it was estimated by scholars that 20 million to 50 million metric tons of e-waste is generated globally every year [25,45-49]. Thus, United States, Western Europe, China, Japan, and Australia are the major countries holding the leading position on the extent of e-waste generation in the world [28,50,51]. Scholars claimed that 50-80% of waste generated in these industrialized nations are probably exported to developing countries like China, India, Nigeria and Ghana [44,48,49,52-53]. Ogungbuyi, Nnorom, Osibanjo & Schluep [38]; UN Comtrade [54] estimated that within the year 2006, 2007, 2008, 2009 and 2010, 2794400, 57050, 46750, 2810900 and 403100 tons of electrical/ electronic wastes were imported to Nigeria respectively. Eyo [40]; Christine [55]; Obaje [37]; Puckett, Westervelt, Gutierrez & Takamiya [32]; Sonny [56] reported that 500 containers of fairly used electrical/electronic appliances are imported to Nigeria every months from Europe with each container holding 500 to 800 computers and monitors representing about 400,000 computers arriving to Nigeria every month. Also, Adediran & Abdulkarim [35]; Amachree [31]; Amanze [57]; Bello, Najib, Umar & Ibrahim [58]; Basel Action Network, BAN (59); Khurrum, Adnan and Xiaozhe [60]; Nnorom & Osibanjo [61]; Percy [62]; Puckett, Westervelt, Gutierrez & Takamiya [32]; Obaje [37] claimed that five million of fairly used personal computers are imported annually into Nigeria through the major sea port of Lagos alone of which 25-75% of these computer wares are unusable junk and unserviceable. In addition to this, Baldé, Wang, Kuehr & Huisman [45]; Chimere, Peter, Martina and Willie [47] reported that in 2014, Nigeria generated about 219 kilo tons of e-waste. Meanwhile, Amoyaw-Osei et al. [63]; Edward-Ekpu [64]; Percy [62] reported that 1,100,000 tons of e-waste are generated in each year in Nigeria. Thus, the internally generated e-waste coupled with imported e-waste positioned Nigeria as one of the countries where greater volume of e-waste is generated in Africa and in the world at large.

Ogungbuyi, Nnorom, Osibanjo & Schluep [38] expressed that the availability of large number of highly trained but low income informal technicians with impressive ability for repairing and refurbishing of used electrical/electronic equipment for local resale has influenced the importation of used electrical and electronic equipment from developed countries like Europe and North America to Africa such as Nigeria. However, following the fact that Nigeria as a nation lacks modern and standardized e-waste recycling facility, - the unusable, nonfunctional and unserviceable e-waste is dumped in several public places and sites spread around the cities and country [39,65].

Hazardous Component of E-waste Material

Analytically, e-waste is made up of certain components like ferrous and non-ferrous metals, and insulators [39]. E-waste items such as battery and Printed circuit boards among others contain primarily metals and non-metals components and most have certain percentage of chromium, lead, copper, nickel, cadmium, and other heavy metals and zinc [26,44,65,66-68]. Scientists affirmed that e-wastes has different hazardous and non- hazardous substances which are broadly consists of ferrous and non-ferrous metals, plastics, glass, wood & plywood, printed circuit boards, concrete and ceramics, rubber and other items [43]. Heacock, Kelly & Asante, et al. [44]; Napoleon and Sinclair [68]; Needhidasan, Melvin & Ramalingam [27] also claimed that e-waste has the content of both toxic and valuable materials in them. Shamsul [69] agreed to this and depicted that most electronic products contain toxic metals of different types which can quickly contaminate the environment when dumped. Thus, electrical/electronic equipment are made from hazardous/toxic elements capable of making e-waste a treat and affecting human and the environmental health in Nigeria [27,32,38,40,60,64,67,69,70-74]. Also, Alake & Ighalo [75] explained that many components of disposed electrical/electronic waste are heinously toxic, non-biodegradable and ecologically debilitating in nature if accidentally released into the environment. In most of the time, e-waste material has severe negative influence on electrical/electronic technicians and the nearby resident, by affecting the air, water and soil around them [38]. Many studies were conducted on identification of e-waste components but none of these studies was specifically focused on hazardous status of e-waste components based on the quantity of abundance in electrical/electronic technicians’ workshops. Thus, part of this study would examine the hazardous status of e-waste components based on the quantity of abundance in electrical/ electronic technician’s workshop.

These toxins will end up in the biological systems of living organisms causing terminal ailments, diseases and death if allowed [75,76]. Also, Omenogo [28]; Yousif [77] submitted that when e-waste is not properly disposed, the toxic substances present in components of electrical/electronic equipment can be harmful to humans and other organisms. Obaje [37]; Terada [65] claimed that the risk from e-waste affects the entire ecosystems and it is a major environmental health risk to wildlife and humans. For instance, Anwesha & Pardeep [78] reported that Guiyu in Hong Kong, a flourishing area of illegal e-waste recycling, is battling with shortages of clean and pure water due to the contamination of water resources by e-waste component. Nnorom & Osibanjo [61]; Sridhar & Bammeke [79] reported that in an industrialized area of Lagos state, some water body were tested to be acidic with PH scale of 3.40. This was attributed to mobility of heavy metals from disposed e-waste items and materials and from ash and cinder resulting from the open burning process, toward water bodies used for domestic purposes [61]. Also, Obaje [37]; Puckett, Westervelt, Gutierrez & Takamiya [32]; Terada [65] reported that 4,000 tons of hazardous e-waste (polychlorinated biphenyi) imported to koko area in Delta state, Nigeria from Italy in 1988 caused many people in the area died of cancerous diseases before it was discovered and repatriated back to source country. It is not doubtful to claim that number of studies were carried out on general hazardous effect of e-waste but none of these studies specifically focused on hazardous effect of e-waste in electrical/electronic service workshop. Thus, this study would investigate the possible effect of electrical/ electronic waste components in electrical/electronic workshop to human and its environment.

Pike Research reported that the volume of electronics e-waste, with increasing adoption of electronic gadgets around the world and ever shorter product life cycles, is expected to double over the next 15 years, from 6 million tons in 2010 to 14.9 million tons, by 2025 [55,80-81]. In another submit, Basel Action Network (BAN) claimed that e-waste generated worldwide has elevated from 9.3 million tons in 2005 to 50 million tons in 2012 [42,30]. Thus, the problem is that only around one tenth of these e-wastes were collected and taken care of [42,48,82- 84] while the remaining 90% become threat to the society and constituting wasting of resources [44,61]. In a real sense, collection of electrical and electronic waste is a sustainable process that maximizes recycling to retain valuable e-waste components in the economy and safely disposes of dangerous components [83-84]. Thus, to get rid of these adverse effects of e-waste that are generated in electrical/electronic service workshop, there is need for proper management of e-waste materials.

E-waste management

Adediran & Abdulkarim [56] submitted that e-waste management is a process of reducing, reusing and recycling of e-waste. Obaje [37]; Waste Management [85] opined that e-waste management is an effective recovery of all reusable materials from old, nonfunctioning, abandoned and disposed electrical/electronic equipment; and safe disposal of the hazardous substances in them to prevent such toxic material from contaminating the environment. Management of e-wastes involves collection, keeping, treatment and disposal of electrical/electronic waste/ scraps in a safe manner to repudiate/prevent human and its environment from harmful effect e-waste [86,87]. Meanwhile, the practices adopted by electrical/electronic technicians in their workshops to manage e-waste generated seem to be illegal, primitive and unsafe. Anwesha & Pardeep [78]; Sepúlveda, Schluep & Renaud [88] claimed that a persistent increase in legal and illegal trade of waste electrical and electronic equipment (WEEE) worldwide has caused equally increasing concern of poor WEEE management techniques. Scholars claimed that unsafe, unregulated and unaccountable collection, processing, and redistribution of old or abandoned electrical/electronic equipment are performed by workers at temporary sites, residences, workshops, and open public spaces [44]. Thus, Heacock, Kelly & Asante, et al. [44]; Solving the E-Waste Problem, StEP, Initiative [89] submitted that general practices of e-waste management among the populaces include using acid baths, burning cables, breaking of e-waste item into smaller parts using forceful approach, and dumping of e-waste materials into an unjustifiable position. Meanwhile, these workers may have embarked on e-waste risky processes and practices due to some challenges they may likely to have been facing such as lack of required knowledge, little or no access to latest technology and personal protective equipment among others [44,90]. However, the studies reviewed expatiated on unsafe practices of public general public regarding e-waste management and challenges they might be encountered while managing on e-wastes management. Thus, none of these studies empirically claimed the common practices of electrical/ electronic technicians regarding e-waste management in Nigeria. One of the focus of this study was to determine the common practice adopted by electrical/electronic technicians to manage e-waste in their workshops and, the challenges which electrical/ electronic technicians were encountering over e-waste management in their service workshops.

Azodo, Ogban & Okpor [88]; Okwesili, Ndukwe & Nwuzor [91] asserted that management of e-wastes focused on efforts of concerned people through conscious and systematic attempt in maintaining and sustaining an aesthetic, economically viable, physically healthy, conducive and safe environment for living. Hossain, Al-Hamadani & Rahman [42]; India Central Pollution Control Board [92]; Nnorom & Osibanjo [61]; Pinto [93] claimed that reclaiming some of the e-wastes materials and recycling them appropriately will mitigate the severe effect of e-waste on living things including plant and animals and their environment. Hossain, Al-Hamadani & Rahman [42] submitted that proper management of these e-wastes is important for the purpose of getting free from hazardous chemicals effect they possessed. Obaje [37]; Opara [94] claimed also that failure to effectively manage e-waste can leads to adverse environmental deterioration, depletion of potentially valuable resource base for secondary equipment and by extension serves as blocking stone for the attainment of sustainable development. The goal three and eleven of sustainable development (agenda 2030) declared in September 25-27, 2015 at United Nation Head Quarters, New York emphasized that quality and healthy living and well-being for all at all ages; and inclusive, safe, resilient and sustainable cities and human settlements must be the ultimate priority of all nations including Nigeria [95-103]. The target for attaining these goals among others includes strengthening the capacity of all countries, in particular developing countries, for early warning, risk reduction and management of national and global health risks; and reduces the adverse per capita environmental impact of cities, including by paying special attention to air quality and municipal and other waste management [101-102]. However, there is need to device appropriate management techniques for e-waste materials in electrical/electronic workshops globally and in Nigeria to be specific. This will go a long way in helping nations including Nigeria to achieve the sustainable healthy environment which goal three and eleven of agenda 2030 of sustainable development is advocating.

Anwesha & Pardeep [76] explained that the appropriate management measures of e-waste include establishment of stringent regulations. United Nations Environment Programme, UNEP, [104] opined that strengthening the political cooperation of a country to promote capacity building among workers and enhance public and private investment on safe and environmentally careful waste management technology will reduce adverse effect of e-waste. Also, Alabaster, Asante & Bergman et al. [105] recognized that bringing poverty to minimum level will alleviate the e-waste challenge in the long-term. Heacock, Kelly & Asante [44] submitted that raise the awareness and regulation of trans-boundary movement and disposal of hazardous and other wastes is a critical means of managing e-waste. Thus, portion of this study would empirically determine the techniques required to manage e-wastes in electrical/electronic service workshops in Nigeria. This is because none of existing studies has ever investigated on techniques for managing e-waste in technician’s workshops. However, the purpose of this study was to advance electrical/electronic waste management techniques among electrical/ electronic technicians’ workshops for sustainable healthy society. Specifically, the study sort to address the following research questions:

What is the hazardous status of e-waste components base on their level of abundance in electrical/electronic technicians’ workshops?

a) What is the hazardous effect of e-waste in electrical/ electronic technicians’ workshops to human and its environment?

b) What are the common practices adopted by electrical/ electronic technicians to manage e-waste in their workshops?

c) What are the challenges which electrical/electronic technicians encountered over e-waste management in their workshops?

d) What is the e- waste management techniques required in electrical/electronic workshops?/p>

Paul [106] claimed that demographic measures typically are used to identify key respondent characteristics that might influence opinion and/or are correlated with behaviours and experiences. Demographic measures include age, type of school, gender, level of academic study, race and educational attainment among other [106-107]. Thus, this study will test influence of qualification, experience, age and occupation on respondents’ opinions on advancement of waste management techniques in electrical/electronic’ workshops for sustainable Nigerian health society.

Hypotheses

a) HA1 Qualification, experience, age and occupation would significantly influence opinion of engineering lecturers and public health officers on hazardous status of e-waste components base on their level of abundance in electrical/electronic technicians’ workshops.

b) HA2 Qualification, experience, age and occupation would significantly influence opinion of engineering lecturers and public health officers on hazardous effect of electrical/electronic waste in electrical/electronic technicians’ workshops.

c) HA3 Qualification, experience, age and occupation would significantly influence opinion of engineering lecturers and public health officers on common practices adopted by electrical/ electronic technicians to manage e-waste in their workshops.

d) HA4 Qualification, experience, age and occupation would significantly influence opinion of engineering lecturers and public health officers on challenges which electrical/electronic technicians encountered over e-waste management in their workshops.

e) HA2 Qualification, experience, age and occupation would significantly influence opinion of engineering lecturers and public health officers on e- waste management techniques required in electrical/electronic workshops.

Materials and Methods

The study adopted Descriptive Survey research design. The study was conducted in Nigeria. Nganzi [108]; Gall, Gall & Borg [109] opined that descriptive surveys research design can be used to collect detailed and factual information that describes an existing phenomenon- their form, actions, changes over time and similarities with other phenomena- from all or a chosen number of the population of the concerned universe. Descriptive survey research design was adopted in this study because information regarding e-waste management was collected from experts using questionnaire. The study sampled one hundred and forty-one participants [110] which included 54 public health officers and 87 engineering lecturers in five Nigerian universities: 20 lecturers from Metallurgical and material engineering, 35 lecturers from Civil engineering and 32 lecturers from Electrical/electronic engineering in Nigeria. The study adopted structured questionnaire as instrument for data collection. The instrument was divided into Part 1 and 2. Part 1 of the instrument was used to receive demographic information of research participants while Part 2 of the instrument was divided into five sections-A, B, C, D and E- to illicit participant responses on hazardous status of e-waste component, hazardous effect of e-waste components, common practice adopted by electrical/electronic technicians to manage e-waste material, challenges encountered by technicians over e-waste management and e-waste management techniques required in electrical/electronic workshops respectively. Questionnaire items were subjected to face and content validity through the help of three experts from two Nigerian universities. The internal consistency of instrument was checked using Cronbach Alfa method. Thus, 0.76, 0.81, 0.69, 0.85 and 0.70 reliability coefficient estimate were obtained for section A, B, C, D and E respectively. Meanwhile, 0.79 was obtained as reliability coefficient estimate for the overall instrument. The instrument was administered using interpersonal contact by researchers and three research assistants. Researcher ensured consent of research participants using interpersonal discussion before the questionnaire is administered and through consent letter attached to the questionnaire. Data obtained were analyzed using statistical software SPSS 22. The research questions were answered using mean, standard deviation and percentage. Meanwhile, t-test and analysis of variance (ANOVA) were used to analyze the data at 0.05 level of significance. 50 percent, being the average percentage value, was considered as cutoff point before any item could be considered as strongly belong to any response category.While the average mean value (cut-off point) decision for section B, C, D and E was 1 2 3 4 5/2=3.0

Presentation of Result

Table 1 shows detail demographic profile of research participants. Specifically, the table illustrated two group of participants- 87 engineering lecturers which comprises of 20 metallurgical and material lecturers, 35 civil engineering lecturers and 32 electrical/electronic lecturers; and 54 public health officers. The participants, in regard to experience distribution, has 9 bachelor’s degree holders, 86 master’s degree holders and 46 PhD holders. The study participants, regarding experience distribution, comprised six participants with 0-9 years of experience, ninety-nine participants with 10-19 years of experience, nine participants with 20-29 years of experience and twenty-seven participants with 30 and above years of experience. Finally, in regard to age distribution, three of participants fell within the age range of 20-30, eleven participants fell within the age range of 31 and 40, eighty four participants fell within the age range of 41-50, thirty nine participants fell between the age ranges of 51- 60 and 4 participants fell within the age range of 61-70.

Data in Table 2 revealed that respondents agreed that all the 34 components of e-waste are hazardous except aluminum. Specifically, lead, mercury, glass and organophosphorus had percentage ratings ranged between 66.6% and 95% under the category of ‘hazardous when slightly abundant’. This depicted that these components are hazardous and can intoxicate the environment irrespective of the volume and quantity. Iron, copper, wood, yttrium, zinc, chlorobenzene and radioactive elements had percentage ratings ranged between 63.6% and 90.8% under the category of ‘hazardous when moderately abundant’. This indicated that these components are hazardous but can intoxicate the environment only when they are fairly or averagely abundant. Also, toner dust, cadmium, brominated flame among other components of e-waste had percentage ratings ranged between 61.0% and 95% under the category of ‘hazardous when largely abundant’. This illustrated that these components are hazardous but can intoxicate the environment only when they are largely or excessively abundant. Meanwhile, aluminum had percentage ratings of 68.8% under the category of ‘not hazardous regardless of state of abundant’. This depicted that aluminum cannot intoxicate the environment regardless of the volume and quantity. Many scholastic reports supported this finding. Reports claimed that over 60% of the total weight of most of e-waste consists of iron, gold, aluminum, copper, lead, mercury, beryllium, cadmium, chromium and brominated flame retardants, of which 2.7% are pollutants [27,110-114] and cause severe hazard to ecosystems with the attendant environmental health risk to wildlife and humans [38,94,115]. The composition of electronic wastes is mostly in various forms and types, containing more than 1,000 different toxic and non-toxic substances [42,116]. Also, gases releases from e-wastes component like polycyclic aromatic hydrocarbons (PAH), brominated flame retardants (BFRs), polychlorinated biphenyls (PCBs) and polychlorinated dibenzo- p-dioxins and furans (PCDD/ Fs) among others affects bio-physical environments and cause detrimental effects to human health [42,117]. Furthermore, scholars reported that some of toxic heavy metals found in e-waste include copper, beryllium, lead, tin, cadmium, brominated flame retardants, antimony, barium, and mercury [25,118]. Obaje [37]; Puckett, Westervelt, Gutierrez & Takamiya [32]; Terada [65] reported that polychlorinated biphenyl (PCB) exported to Koko area of Delta State, Nigeria in June 1988 was hazardous and caused death of cancer to people of the community.

Table 3 showed that the hazardous effect of e-waste in electrical/ electronic workshops stood at mean value of (4.49±0.51). Meanwhile, the mean rating for each item stood at mean values ranged between (4.14±0.35) and (4.63±0.48). This depicted that all the 25 items had mean ratings above the cut-off point of 3.0 and were considered by respondents as hazardous effect of e-waste in electrical/electronic technicians’ workshops to human and its environment. United Nations Environment Programme [49] supported this result and herein reported that failure to care for solid wastes including e-waste incurs a severe penalty, later, in the form of resources needlessly lost, foul odours and unsightliness and contamination of air, water, and soil resources. Individuals who live near or on disposal sites, are infected with gastrointestinal parasites, worms, and related organisms [49]. Furthermore, study found that 36.3% of 1,000 women living near the informal recycling sites experienced stillbirths in the Sylhet region of Bangladesh and 64% had hearing and/or vision problems [42,119-121]. Also, burnt e-wastes produce smoke, dust or carbon particles from toners consist of carcinogens and other hazardous chemicals which causes severe inflammations and lesions including many respiratory, lung and skin cancer and diseases [68]. 

Table 4 showed that the common practices adopted by electrical/electronic technicians to manage e-waste in workshops stood at mean value of (4.12±0.61). Meanwhile, the mean rating for thirteen items (item 1, 2, 3 4, 5, 6, 7, 8, 9, 10, 11, 13 and 14) stood at mean values ranged between (3.19±0.51) and (4.56±0.50). This proclaimed that all thirteen items had mean ratings above the cut-off point of 3.0 and were considered by respondents as common practices adopted by electrical/electronic technicians to manage e-waste in workshops. Meanwhile, item 12 had rating value (2.34±0.91) bellow the cutoff value of 3.00 and was not considered by respondents as common practices adopted by electrical/electronic technicians to manage e-waste in workshops. The scholars Needhidasan, Melvin & Ramalingam [27]; Sivakumaran [68] supported this finding wherein reported that dismantling of e-wastes takes much labor, in countries like China and in some parts of India where big quantity of tons of e-wastes are junked, dismantled, shredding, tearing and burning. Needhidasan, Melvin & Ramalingam [27]; Sinha-Khetriwal [73] reported that in Mumbai metropolis alone, people throw away 19,000 to 20,000 tons of electronic waste every month. Babatunde [25] reported that uncontrolled burning, disassembly and disposal which are common in the society can cause environmental and health problems. Also, Edward-Ekpu [64] reported that in Nigeria, a lot of e-waste are also stockpiled in offices and homes.

Table 5 showed that the challenges which electrical/electronic technicians encounter over e-waste management stood at mean value of (4.62±0.44). Meanwhile, the mean rating for each item stood at mean values ranged between (4.40±0.49) and (4.94±0.23). This depicted that all the 10 items had mean ratings above the cut-off point of 3.0 and were considered by respondents as challenges which electrical/electronic technicians encounter over e-waste management in workshops. Thus, this result was supported by scholars and scientist in the field of waste management. Obaje [37] submitted that e-waste is a major problem in Nigeria today because there is very low level of awareness of the hazards and health risk associated with e-waste. Needhidasan, Melvin & Ramalingam [27] reported that people who are working with e-waste sector are the urban poor with very low literacy levels and hence have very little awareness regarding the hazards of e-waste toxins. Babatunde [25] reported that one of the challenges facing e-waste management in Nigeria is lack of public awareness by manufacturers and consumers on the inherent dangers of handling and trading in e-waste and lack of a comprehensive e-waste management regulation or legislation in Nigeria to regulate, control and prohibit the flow of used consumer electronic products within Nigeria. E-waste collection in Nigeria is not organized because there are no collection centres for e-waste materials and this however caused most of e-wastes to be dumped along with other wastes [64].

Table 6 revealed that the e-waste management techniques required in electrical/electronic workshops stood at mean value of (4.43±0.49). Meanwhile, the mean rating for each item stood at mean values ranged between (4.03±0.58) and (4.96±0.20). This proclaimed that all the 34 items had mean ratings above the cut-off point of 3.0 and were considered by respondents as e-waste management techniques required in electrical/electronic workshops. The reports of past research supported the present findings. Manfred [122] supported this finding and herein reported that waste management service is falling too short of the desired level of efficiency and satisfaction resulting in problems of health, sanitation and environmental degradation due to absence of serious efforts from necessary authorities. Thus, Needhidasan, Melvin & Ramalingam [27] reported that technical and policy-level interventions, implementation and capacity building and increasing the public awareness can convert this challenge of massive e-waste generated into an opportunity. Babatunde [25] recommended that government should establish regulatory framework, through relevant agencies to manage e-waste and permanent e-waste collection facilities should be provided at strategic locations and usage of such facilities should be enforced. Ewuim, Akunne, Abajue, Nwankwo & Faniran [39] submitted that mass education and awareness should be created by regulatory bodies and government on inherent dangers of poor e-waste management on the environment.

Table 7 shows the summary of analysis of t-test and one-way between-groups analysis of variance that was conducted to explore the influence of qualification, experience, age and occupation of public health officers and engineering lecturers on their response to research questions. Participants were divided into three, four, five and two groups according to their qualification, experience, age and occupation respectively.

Hypothesis 1

Thus, qualification statistically influenced the response of engineering lecturers and public health officers on hazardous status of e-waste components in electrical/electronic workshops base on level of abundance: F (2,138) = 6.688; Sig.= 0.002. Experience statistically influenced the response of engineering lecturers and public health officers on hazardous status of e-waste components in electrical/electronic workshops base on level of abundance: F (3,137) = 8.018; Sig.= 0.000. Age statistically influenced the response of engineering lecturers and public health officers on hazardous status of e-waste components in electrical/electronic workshops base on level of abundance: F (4,136) = 24.228; Sig.= 0.000. Meanwhile, occupation did not statistically influence the response of engineering lecturers and public health officers on hazardous status of e-waste components in electrical/electronic workshops base on level of abundance: F (139, 90.976) = 0.232; Sig.= 0.631.

Hypothesis 2

Qualification statistically influenced the response of engineering lecturers and public health officers on hazardous effect of e-waste in electrical/electronic technicians’ workshops: F (2,138) = 7.384; Sig.= 0.001. Experience statistically influenced the response of engineering lecturers and public health officers on hazardous effect of e-waste in electrical/electronic technicians’ workshops: F (3,137) = 11.573; Sig.= 0.000. Age statistically influenced the response of engineering lecturers and public health officers on hazardous effect of e-waste in electrical/ electronic technicians’ workshops: F (4,136) = 11.365; Sig.= 0.000. Meanwhile, occupation did not statistically influence the response of engineering lecturers and public health officers on hazardous effect of e-waste in electrical/electronic technicians’ workshops: F (139, 110.082) = 0.145; Sig.= 0.704.

Hypothesis 3

Qualification did not statistically influence the response of engineering lecturers and public health officers on common practices adopted by electrical/electronic technicians to manage e-waste in the workshops: F(2,138)= 0.556; Sig.= 0.575. Experience did not statistically influence the response of engineering lecturers and public health officers on common practices adopted by electrical/electronic technicians to manage e-waste in the workshops: F(3,137)= 0.700; Sig.= 0.553. Age did not statistically influence the response of engineering lecturers and public health officers on common practices adopted by electrical/ electronic technicians to manage e-waste in the workshops: F(4,136)= 0.923; Sig.= 0.453. Also, occupation did not statistically influence the response of engineering lecturers and public health officers on common practices adopted by electrical/ electronic technicians to manage e-waste in the workshops: F(139,104.969)= 0.627; Sig.= 0.430.

Hypothesis 4

Qualification statistically influenced the response of engineering lecturers and public health officers on challenges which electrical/electronic technicians encounter over e-waste management: F(2,138)= 9.809; Sig.= 0.000. Age statistically influenced the response of engineering lecturers and public health officers on challenges which electrical/electronics technicians encounter over e-waste management: F(4,136)= 4.856; Sig.= 0.001.Meanwhile, experience did not statistically influence the response of engineering lecturers and public health officers on challenges which electrical/electronic technicians encounter over e-waste management: F(3,137)= 0.319; Sig.= 0.812. Also, occupation did not statistically influence the response of engineering lecturers and public health officers on challenges which electrical/electronic technicians encounter over e-waste management: F(139, 113.799)= 0.084; Sig.= 0.773.

Hypothesis 5

Qualification statistically influenced the response of engineering lecturers and public health officers on e-waste management techniques required in electrical/electronic workshops: F(2,138)= 24.196; Sig.= 0.000. Age statistically influenced the response of engineering lecturers and public health officers on e-waste management techniques required in electrical/electronic workshops: F(4,136)= 17.938; Sig.= 0.000.Meanwhile, experience did not statistically influence the response of engineering lecturers and public health officers on e-waste management techniques required in electrical/electronic workshops: F(3,137)= 0 .781; Sig.= 0.507. Also, occupation did not statistically influence the response of engineering lecturers and public health officers on e-waste management techniques required in electrical/electronic workshops: F(139, 113.799)= 0.095; Sig.= 0.757. Omenogo [28], while comparing the practices of two tertiary institutions in Nigeria on e-waste management, reported that institution was not a determining factor on whether a department keep records of discarded electronic equipment and records of stored out of use electronic items. Ohajinwa, Peter, Martina & Willie [123] reported also that health risk awareness level of the e-waste workers was significantly lower compared with their counterparts in the same informal sector. Thus, this study has commonalities with those previous studies.

Conclusion

The study investigated advancement of electrical/electronic waste management techniques among electrical/electronic technicians’ workshops for sustainable healthy society. The study uncovered the hazardous status of many composition of electronic waste materials based on the level of abundance. The study also revealed empirically the hazardous effect of e-waste materials in electrical/electronic workshops. Also, study confirmed the common e-waste practices among electrical/electronic technicians in their workshops and the challenges they are facing on e-waste management. Finally, this study discovered e-waste management techniques required for managing e-waste materials in electrical/electronic workshops. This study was geared purposely to reconstruct our society for sustainable and conducive living.

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Wednesday, January 27, 2021

Probiotics Use in Horse Feeding - Juniper Publishers

 JOJ Sciences - Juniper Publishers


Abstract

Probiotics are live microorganisms, offered in animal feed at adjusted doses, providing improvements in host health. Several studies have confirmed the yeasts effect on the health, digestibility coefficient, and performance of horses, that way, the Saccharomyces cerevisiae yeast can become an important probiotic additive for equines besides reducing the incidence of colic in these animals.

Keywords: Equine; Saccharomyces cerevisiae; Yeasts; Intestinal microorganisms; Supplementation; Additives normal; Microbiota; Intestinal environment; Digestive tract; Horse feeding; Microorganism; Food digestibility; Gastric environment; Antimicrobial properties; pH; Temperature; Probiotics

Introduction

In equidae, probiotics are used trying to establish a desirable balance among intestinal microorganisms, especially that beneficial. For these benefits, supplementation of these additives in the diet creates an expectation, after use, to provide viable and suitable microscopic organisms for adaptation in the intestinal environment, acting in a beneficial way in the balance of the normal microbiota of the digestive tract, increasing zootechnical performance and/or preventing pathologies of the digestive tract [1]. Therefore, it is essential to evaluate in a more specific way the use of yeasts in the horse feeding as potential probiotics for the specie, considering there are indications of these acting positively on the food digestibility and health of the horses.

Probiotics Characterization

For a microorganism to be considered a good probiotic must have some attributes such as being able to survive the gastric environment, antimicrobial properties, higher growth rate than its elimination by intestinal peristalsis, able to adhere to mucus and epithelial cells, and it is essential to be produced and remains viable for long periods. The first three mechanisms are usually attributed to lactic acid producing bacteria, while the latter two are more yeast specific [1].

The characteristics attributed to probiotic microorganisms are related to the production of substances that suppress pathogens or prevent their growth, competition with them for adhesion sites and nutrients, preventing they to produce toxins as well as inhibiting their action; to be innocuous to the host to not cause any disease, and it must be natural to the digestive system, being able to resist to this environment, mainly to changes in pH and temperature [2].

Several microorganisms are currently used as probiotics, the most common of which are Lactobacillus, Bifidobacterium, Streptococcus, and live yeasts such as Saccharomyces cerevisiae [3]. Saccharomyces cerevisiae yeast has good results, promoting intestinal microbiota balance, which can be observed in an experiment where it was used a dose of 2g/day of yeast for 4-year-old mini-horses, showing balance in the intestinal microflora, and with changes in the bacterial population [4].

Effect of the Saccharomyces cerevisiae Use on the Diet of Horses

Probiotics are widely used for equines in the recovery of animals undergoing some type of stress, such as deficiency in colostrum consumption, stress generated by the weaning process, during transportation with significant water and food restrictions, abrupt climatic changes, chronic morbidities due to mismanagement such as laminitis or gastrointestinal diseases, supplements for animals with low body score, and long-term therapy in order to circumvent the use of antibiotics [5]. In addition, it is used to try to establish a desirable balance between intestinal microorganisms, especially those beneficial. These additives have become a differentiated option due to their attributes, as their ability to reduce infectious pathologies and, consequently, reduce the use of antibiotics [6], as well as to reduce cases of intestinal obstruction. They may be offered to animals in a variety of ways, such as powders, pastes, gels and most often orally, mixed with food during the meal or supplied with water [7].

Among these probiotics, Saccharomyces cerevisiae has potential to be used as a probiotic in equines, since research in other species of animals has shown positive effects [8,9]. In weanling foals, the use of Saccharomyces cerevisiae yeast at 5 g/day showed positive results in hemicellulose digestibility [1]. In diets with low-quality bulky food supplemented with this yeast, the use was approximately 9% better, with an increase in the digestibility coefficient values when compared to the yeast-free diet FURTADO [10]. Similar results were observed when supplementing 20 g of the probiotic Saccharomyces cerevisiae per day for horses under training, with an increase in hemicellulose digestibility of 4.1% [11]. Thus, in order to improve the digestibility of the fibrous portion and increase the energy available to the animals, the diet can be increased with these probiotic additives

Conclusion

The information presented demonstrates that the use of the probiotic Saccharomyces cerevisiae improves the actions of the intestinal microbiota, allowing the greater degradation of the fibrous components of the diet, which predisposes better health conditions to the supplemented animals.

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Thursday, January 21, 2021

Tyrosine on Cutaneous Pigmentation - A New Therapy Line for Vitiligo - Juniper Publishers

 Dermatology & Cosmetics - Juniper Publishers


Abstract

The topical effect of tyrosine in altering skin pigmentogenesis was studied using melanin pigment producing strain of Cryptococcus neoformans. Although the biochemical pathway is different in C. neoformans, where phenol oxidase enzyme is involved unlike tyrosinase as in the case of mammalian cell, still the findings prove the method to be reliable and reproducible. Peri-cellular absorption of tyrosine and subsequent melanogenesis were observed in treated cell of C neoformans. Further the quality of pigment produced in the cell of the organism was different from phenol oxidase induced pigment. The pigment thus offered protection to the cells from UV damage. The observation based study on tyrosine based cream in human skin and subsequent sun exposure resulted in minimal erythema and delayed pigment darkening indicating the underlying melanogenesis process. Further, the absence of erythema suggests the possible melanogenesis activation at sub-dermal level and its effect in protecting the skin from sun damage. The clinical usefulness of topical tyrosine and other scientific details are discussed in the paper.

Keywords: Vitiligo; Tyrosine; Phenoloxidase; L-Dopa; Guizotia Abyssinica; Uv Treatment; Pigmentogenesis

Introduction

A rapid, simple, less time consuming, cost effective and finally a reliable and relatable method for screening agents/ingredients and developing a formulation for the management of vitiligo and hypopigmentary disorders of the skin is greatly lacking [1]. Although cell culture methods are available and are used widely for the above purpose, but still the extrapolation of the cell culture findings to predict the possible treatment outcome based on the cell culture assay of agents/ingredients is quite tricky, confusing and in most cases, not relatable [2]. Several microorganisms are known to produce melanin like substance for their defense and the fungi -Cryptococcus neoformans is the best example for melanin synthesis [3]. In general, the microbes synthesize melanin mostly via phenoloxidase but in mammalian system, tyrosinase replaces the role of phenoloxidase. In any case L-DOPA and tyrosine are the precursor molecules for the biosynthesis of melanin [4]. The extracellular supplementation of tyrosine into the growth medium of C.neoformans was found to accelerate the biochemical process of melanogenesis. Further the melanin thus formed was found to accumulate in the cells but a distinct capsule formation around the cell was absent. C. neoformans being a ‘simple life unit’; any changes in the cells of C.neoformans therefore can be easily co-related to the mammalian system [5].

In the case of cell culture assay, where the melanocytes are used but the melanocytes always exist only as an individual cell unit and may not reflect the possible response of the skin in to [6]. Therefore the study outcome that is obtained with cryptococcal cells is more relatable to the cutaneous response than the use of melanocytes. In our present study we have established the extracellular tyrosine induced melanogenesis in cryptococcal cells and such cells are relatively protected from UV induced cell death/mutation. The interesting feature was that the cells grown in tyrosine supplemented media showed greater resistance to UV treatment than the cells grown in guizotia abyssinica agar supplemented with creatinine. Vitiligo being an autoimmune disorder with progression tendency, palliative, tyrosine based therapy may be effective for long term purposes even if the treatment outcome is relatively sluggish [7,8]. In the case of progressive vitiligo, tyrosine based treatment even if it retards the progression of hypopigmentation by 50% still such treatment would be highly valuable. In the present study we report the use of extracellular supplementation of tyrosine and its usefulness in the treatment of vitiligo. Details are presented in the paper.

Materials and Methods

Growth of C.neoformans in tyrosine supplemented media

Sabouraud’s dextrose agar supplemented with 3 different concentrations of tyrosine such as 0.1, 0.2, 0.3 μg/ml respectively were used for the experiment. The freshly grown C.neoformans were inoculated and incubated in the above media condition for 7 days and then observed the colony and the cells for melanin, both macroscopically and microscopically [9,10].

Growth of C.neoformans in guizotia abyssinica agar with creatinine

Cryptococcal cells were grown in guizotia abyssinica agar supplemented with creatinine and incubated for 7 days. After 7 days the colony characteristics and cells were observed for melanin as described above [11,12].

Response of cells to UV

C.neoformans grown in tyrosine supplemented media and from guizotia abyssinica agar supplemented with creatinine were grown again in sabouraud’s agar and were then immediately were exposed to UV for 5 minutes. The number of colonies grown was counted and the melanin synthesized in the cells vis-à-vis UV protection was ascertained.

Melanin quantification

Melanin produced in C.neoformans cells grown in tyrosine supplemented media and guizotia abyssinica agar supplemented with creatinine was quantified using a standard procedure. Approximately 109 cells grown in different experimental setup were pelleted by centrifugation at 1,000 g for 5 min and then the cells were washed with phosphate-buffered saline. After recentrifugation as described above, the supernatant was decanted, the cells were precipitated again, re-suspended in distilled water and 1 ml of ethanol – ether 1:1 (vol/vol) was added to remove the artifact other than melanin. Cell wall lysis procedure was also followed. The insoluble melanin in ethanol–ether was resuspended and centrifuged at 3,000 g for 5 min, the precipitate was dissolved in 1 ml of 1 N NaOH/10% dimethyl sulfoxide at 8 0C for 30 min. The absorbance was measured at 470 nm, and the melanin synthesized in treated and control cells were calculated.

Effect of topical tyrosine on melanogenesis and erythema formation in human volunteer

Five human volunteer were chosen for the study. The cream with tyrosine (0.2%) and without tyrosine was applied over the volar forearm region at 2mg/cm2 separately. Then the volar forearm region was exposed to sunlight for 15minutes and the extent of erythema formed in the treated and control sites and between products with and without tyrosine was recorded immediately after exposure and the pigment production (tanning) in the above experimental set up also recorded after 24 hours [13,14].

Results

Growth of C.neoformans in tyrosine supplemented media

The cells grown in tyrosine supplemented media showed the melanin synthesis which is directly proportional to the concentration of tyrosine used (Figure 1-4). C.neoformans grown in guizotia abyssinica agar supplemented with creatinine did produce intense pigmentation. Microscopic appearance of C.neoformans cells grown in tyrosine supplemented media showed the accumulation of melanin to be higher in cells grown in tyrosine supplemented media with cell wall distinctively with devoid of capsule formation and cell enlargement.

Response of cells to UV

We have used 500 CFU’s of C.neoformans for UV exposure. UV exposure in the case of the organism grown in SDA showed a significant reduction in the colonies. Only 13/500 could grow after UV exposure. Whereas the UV untreated plate yielded 496/500 CFU’s. In the case of organism grown in tyrosine supplemented media when exposed to UV, 76% CFU’s could withstand the UV treatment with only 7.8% of the cells that were grown were mutant colonies. In the case of organism grown in Guizotia abyssinica agar supplemented with creatinine media when exposed to UV yielded only 24% of cells with 93.3% of colonies were mutant.

Melanin quantification

Tyrosine treatment significantly increased the melanogenesis in a concentration dependent manner. The C.neoformans grown in guizotia abyssinica agar supplemented with creatinine also showed melanin synthesis.

Effect of topical tyrosine on melanogenesis and erythema formation in human volunteer

None of the 5 volunteers treated with tyrosine incorporated cream showed erythema response to sun exposure whereas all of them showed varying degrees of tanning after 24 hours.

None of the 5 volunteers treated with the cream devoid of tyrosine showed any tanning response after 24 hours. However all of them showed varying degrees of erythema within 15 minutes of sun exposure.

Discussion

Our present investigation has brought out a new treatment possibility for vitiligo and hypopigmentary disorders of the skin. Tyrosine is although known as the fundamental unit of skin pigment called melanin but still tyrosine is not the sort after treatment option for vitiligo. It is always assumed that amelanotic condition or the ability of the melanocytes to produce the pigment or due to the enzymatic or the biochemical pathway aberrations as the causes for vitiligo. Substrate availability in excess may sometime offer solution to the problem. However which patient may require such treatment option is difficult to predict because the etiopathology of vitiligo and hypopigmentary disorders of the skin is too complex. Therefore simultaneously adopting several treatment options to address various pathological triggers and correcting each of them is necessary. In the present study, we have explored how the extracellular supplementation of tyrosine would increase pigmentogenesis in C.neoformans. In our earlier study we have already established the use of C.neoformans in the screening and identifying the skin pigment modulators as effective as melanocytes based screening methodology.

The advantage of using C.neoformans is that the C.neoformans is a eukaryotic microorganism and also it represents the life in totality. Whereas, the melanocytes are one of the cells in the skin and it requires several direct and indirect communication network with other cells and the feedback mechanism to function. Therefore the scope of predicting the therapeutic usefulness of any compound or agent tested by C.neoformans will have a higher relatability than melanocyte or tyrosinase enzyme based screening outcome. The C.neoformans cells treated with varying concentration of tyrosine has significantly increased the melanogenesis, melanin accumulation and cell wall conspicuous. Further the pigmentogenesis in C.neoformans had occurred in 7 days as against 14 days in the case of guizotia abyssinica agar supplemented with creatinine. Interestingly the capsule formation was fairly less in C.neoformans cells grown in tyrosine supplemented media than guizotia abyssinica agar supplemented with creatinine. The formation of capsule is linked more with virulence and the melanoid formation is linked with the survival of cells.

The pigmentogenesis with poor capsule formation therefore suggests that the tyrosine may be serving only as substrate for the pigmentation and other than that the tyrosine may not alter the virulence mechanism of organism. The absorption of tyrosine from the media by C.neoformans cells clearly suggests the possibility of dermal absorption of tyrosine because the cell wall architecture of C.neoformans is quite impermeable for the easy entry of agents. Therefore a cream with tyrosine may offer better relief to the problems of hypopigmentary disorders; where the substrate abundance is sufficient to promote pigmentation. The microscopic examination of the cells clearly reiterates that tyrosine induces melanogenesis in a dose dependent manner with not much alteration either in the cell size or in the cell wall armamentarium. The cells grown in different growth conditions such as SDA, tyrosine supplementation and guizotia asyssinica agar were exposed to UV to check how the melanogenesis in the cells offer protection from UV.

The cells grown in SDA could not withstand UV exposure and hence majority of the cells could not survive and only a limited number of mutant colonies alone could survive. In the case of tyrosine treatment about 70% of the cells survived UV exposure clearly suggesting that the melanin produced by the cells may be responsible for the UV protection. The countercheck measures on mutant colonies are also revealed only the above possibility. Whereas the cells grown in guizotia abyssinica agar supplemented with creatinine could not withstand UV exposure despite high melanin synthesis. We presume that the phenol shock in the guizotia abyssinica agar may have made the cells vulnerable to UV further the melanin synthesis in the cells in the above media may not be uniform and hence the survival rate of cells vis-àvis mutant formation were almost equal. The quantification of melanin assay has revealed that C.neoformans grown in tyrosine supplemented media yielded pigment which is slightly less blackish than the C.neoformans cells grown in guizotia abyssinica agar supplemented with creatinine.

C.neoformans produces pigment through a different biochemical pathway than the mammalian cells. Phenol oxidase enzyme in C.neoformans which is an analogue of tyrosinase in a mammalian system results in pigment production in C.neoformans. The reason for the poor survival rate of C.neoformans when exposed to UV despite melanin synthesis may be due to the phenol shock in guizotia abyssinica agar medium. The qualitative aspect of the pigment produced by the cells grown in tyrosine supplemented media was significantly different from that of the cells grown in guizotia abyssinica agar supplemented with creatinine; wherein the melanin was densely colored may be due to the capsule inclusion. We presume that the tyrosine may trigger melanogenesis although through phenoloxidase pathway but may be limiting the events just to pigmentogenesis. Whereas the cells grown in guizotia abyssinica agar supplemented with creatinine may pose ‘phenol threat’ to the cells and hence the production of pigment, capsule formation and other associated changes may be taking place in the cells and hence the resultant product is quite dark and dense but still not protecting the cells from UV exposure. Subsequent to the above experiment, we also tried to answer the question of whether topical tyrosine when results in pigmentogenesis also alter the inflammatory responses.

We have used two sets of creams viz., one with tyrosine and without tyrosine. The solar effect on tyrosine supplemented and the cream devoid of tyrosine was checked by applying the creams over the volar forearm region in volunteers and then expose to sunlight. To our surprise we found that tyrosine supplemented cream did not cause erythema formation in response to sun exposure, however resulted in delayed pigment darkening (tanning) after 24 hours. Tyrosine doesn’t filter down UV A selectively and hence the delayed pigment darkening necessarily cue only melanogenesis due to abundant substrate availability. The sun exposure has triggered the evolutionary instinct of the skin to produce pigment and when the substrate was available the pigmentogenesis has taken place. The process involved in melanin pigmentation might have protected the skin and hence no erythema formation was observed in tyrosine supplemented cream was when used.

The above findings further suggest that tyrosine may not only augur melanogenesis in the skin but also may permeate through skin when applied topically. However the delayed pigment darkening in volar forearm region treated with tyrosine supplemented cream disappeared by 48hrs clearly indicating the melanogenesis event was only responsible for tanning and not the dermal protective response to sun exposure. The cream devoid of tyrosine did not offer sun protection and hence the erythema was obvious within 15 minutes of sun exposure. But no immediate or delayed pigment darkening has occurred. The characterization of melanin by Electron Paramagnetic Resonance spectra (EPR) data not included showed that the pigment produced by C.neoformans cells grown in tyrosine supplemented media was distinctly different from that of guizotia abyssinica agar supplemented with creatinine. Topical tyrosine may offer the much needed therapeutic benefit to vitiligo patients as revealed by our study.

However such positive response is possible only in those patients who may suffer from the scarcity of the substrate of melanin- Tyrosine. Differentiating the different etiological causes of vitiligo and accordingly modifying the treatment may not be possible in the case of vitiligo and hence all treatment options such as increased substrate availability, promotion of tyrosinase activity, increased dendrite number and length in melanocytes and accelerating the melanin transfer mechanism to keratinocytes are required. Immunosuppressive agents also required if the active predation of melanocytes by the self -immune defense mechanism were the cause for the pigment loss. Our study has brought out a ray of hope for the treatment of vitiligo. Considering the tyrosine being the last biochemical molecule (amino acid) with decent bio-absorption through skin tyrosine based treatment option may hold key to vitiligo treatment in near future. The cream partitioning co-efficient is necessary for the rapid and sustained release of tyrosine from the cream and therefore it is not just the use of tyrosine alone may offer the result but the formulation science also plays a significant role.


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Wednesday, January 20, 2021

Dependence of Chlorophyll Content in Leaves from The Light Regime, Electromagnetic Fields and Plant Species - Juniper Publishers

 Horticulture & Arboriculture - Juniper Publishers  


Introduction

The productivity of photosynthesis of green plants is determined mainly by their genetics and strongly depends on temperature, nutrient medium and light regime Polevoj [25]; Andrianova & Tarchevskij [2]. These factors determine the structure and efficiency of the Photosynthetic Apparatus of the plant (PSA). The key functional elements of PSA are a and b forms of the Chlorophyll (Chl) Senge [28]; Kholmanskiy & Smirnov [16] and water, which plays the role of a dynamic matrix and active metabolite Polevoj [25]; Kholmanskiy & Tilov [13]; Aksenov [1]. Water sensitizes PSA by forming complexes with Mg and chiral centers Chl Kholmanskiy & Smirnov [16] and other molecules in the composition of PSA Senge [28]; Kholmanskiy [14]; Kholmanskiy [15]; Zaytseva & Sitanskaya [37]. The abnormal thermodynamic properties of water optimize the physics of cold stratification of seeds at a temperature of about 4°C Kholmanskiy [15] and minimize the energy of seed germination and photosynthesis in the temperature range 15-25°C Polevoj [25]; Kholmanskiy [15]. The photo- and thermophysics of Chl substantially depend on the Mg ion in the center of porphyrin cycle and the electronic nature of substituents in it Senge [28]; Kholmanskiy & Smirnov [16]. In principle, chlorophyll can play the role of a marker of the total Mg content in a plant and therefore can be considered an adequate characteristic of the nutritional value of a plant product.

The photochemistry and photo physics of PSA are determined primarily by the electronic structure of the ground and excited electronic states of Chl. A significant difference in the biophysics of chlorophyll Chl a and Chl b Tyutereva & Ivanova [35] is caused by the replacement of the CH3 group by the electron-acceptor and proton donor groups of CHO in the 7th position of pheophytin (Figure 1). In Frese [9]; Kholmanskiy & Smirnov [16], the electronic nature of the ground and lower excited states of Chl was attributed to states with charge transfers whose dipoles are oriented along the mutually orthogonal X and Y axes of the Chl molecule. The high dipole moments of the excited states of Chls initiate electron and proton transfers from other PSA components and these reactions can accelerate the kinetics of photochemical reactions in PSA. It was suggested in Kholmanskiy [18] that micro polarization of PSA promotes proton diffusion in leaves and intensifies the extraction of micro and macro cell ions by a plant. Mg and Chl greatly increase the nutritional and medicinal value of cultivated plants, both leafy and fruit-bearing. In Kholmanskiy [17-18] it was established that the efficiency of Mg extraction by plant leaves of cultivated plants depends on their species and responds to changes in the irradiation spectrum. It can be assumed that a similar dependence should be observed for the content of Chl in plant leaves. To deepen understanding of the biophysical relationship between plant productivity and Chl we compared the total content of a+b Chl and Mg in raw samples of leaves of a number of cultivated plants and steppe grasses. We analyzed the dependence of the Chl content in the leaves of cultivated plants on the light regime, and also compared the effects on photosynthesis of Chl a and Chl b in plantain and wheat seedlings of an electromagnetic industrial Novichkova & Podkovkin [23]; Shashurin [27] and an electrostatic vortex field Therapeutic reel Mishina [33].

Results and discussion

The empirical data on the content of chlorophylls a and b in cultivated plants and grasses were taken from books and works published by us and other authors. Table 1 shows the total content of Chl a+b (hereinafter [Chl]) and the ratio a/b for raw samples of plant leaves. The determination of [Chl] in the works was carried out according to the methods described in Lichtenthaler & Buschmann [21]. In Bohn [3] [Chl] was determined using liquid chromatography, and the Mg content in Chl was calculated based on its mass fraction of ~2.7% in a and b Chl. In this work, we compared the concentrations of total Mg and Mg in the composition of Chl (Table 2).

A large scatter of [Chl] values in different works is caused by differences in both their measurement methods and the growing conditions of the same plants. The [Chl] values determined for dry samples were recalculated for the wet state using a drying coefficient (k). It was evaluated by determining the weight of a fresh sample and after drying it at a temperature of no higher than 40°C. For vegetable crops (tomato, cucumber), leaf (lettuce, greens) and steppe grasses their k were: 8.5; 15-20 and 6-7, respectively. At the same time, the proportion of water in raw samples was determined by the ratio (k-1)/k and was equal to: 88%; 93-96% and 85-86%, respectively.

The Table 1 shows [Chl] values for plants growing in natural and greenhouse conditions. In the latter case, the age of the samples (weeks) and the light regimes were varied. The irradiators included various combinations of Blue (Bl), Green (Gr), Red (Rt) Light Emitting Diodes (LED1 and LED2) Kholmanskiy [18] and 400W high pressure sodium (SL) and mercury (ML) lamp. Their emission spectra and the absorption spectrum of Chl are shown in Figure. In some works, the LED composition included a diode emitting in the far-red region of the spectrum (FRt) with a maximum at 730nm. Plant species in Table 1 are divided into three groups: vegetables, cereals, berries (I); lettuce and greens (II); grasses (III). The mean values of [Chl] in the II and III groups are close and 1.6 times less than in the I group. At the same time, [Chl-b] in the I-st III-th is 2 times less than [Chl-a], and in the II-th one 3.3 times. The value of [Chl] in the first and second groups of plants reaches a maximum at 3 weeks of growth and depends on the spectral composition and intensity of irradiation. The Сhl content decreases when the irradiation intensity is exceeded by the norms of the optimal light regime Dalke [7]. Moreover, the Chl photo destruction reaction can contribute to the negative effect Kholmanskiy & Smirnov [16]. The efficiencies of Chl biosynthesis in the leaves of plants of group I are close when the plants are irradiated with LED irradiators and the sun and are higher than when irradiated with SL. This result can be explained by the good overlap of the Rt emission bands of LED1 and LED2 with the first absorption band of Chl a and b (Figure 2). In group II, the dependence of the efficiency of Chl biosynthesis on the irradiation spectrum is less pronounced. Chl biosynthesis is limited by the efficiency of the plant’s extraction of the Mg, which depends on the type of plant and the irradiation spectrum Kholmanskiy [18]. Table 2 shows the total content of Mg, Chl, and Mg in Chl ([Mg-Chl]) in the leaves and fruits of plants of the first and second groups, for samples grown under irradiation ML. From these values, the concentration ratio of total Mg to Mg in the composition of Chl (Mg/[Mg-Chl]) was calculated.

The obtained relations Mg/[Mg-Chl] indicate that the total Mg content in plants significantly exceeds [Chl], and this disproportion in plants of the 1st group is 4-5 times greater than in plants of the 2nd group. This is due to the fact that Mg is included in the active center of the enzyme, providing assimilation of CO2 Polevoj [25] and also participates in the activation of many other complexes Senge [28]; Shkol’nik [29]; Sukovataya [32]. In addition, the genetics of cucumber and tomato, unlike lettuce, includes programs for the stages of flowering and fruiting with their specific biochemistry and bioenergy Shkol’nik [29]; Tikhomirov, Sharupich [34]. A significant contribution to the intensification of photosynthesis and enzymatic reactions in animal and plant organisms is made by the magnetic isotope 25Mg Buchachenko [5] the content of which in natural magnesium is 10%. Apparently, 25Mg in the composition of enzymes plays an important role in the functioning of the phytochrome and cryptochrome PSA systems, activating magnetically sensitive dark reactions of radical ion pairs Evans [8]. Chl charge transfer states in dark reactions relax with the formation of long-lived excitons Frese [9]. Their participation in the work of PSA can, in principle, determine the dependence of the efficiency of photosynthesis on external electromagnetic and electric fields. To verify this assumption, we compared the dependence of the content of pigments - Chl a, Chl b and carotenoids (k) in maple leaves growing at a distance of 0 to 1000m (control) from 110kV power lines Novichkova & Podkovkin [23] and in the leaves of plantain seedlings, grown in an Electromagnetic Field (EMF) with a frequency of 50Gz and different intensities Shashurin [27], as well as wheat seedlings growing in an electrostatic vortex field with a frequency of 300kGz Therapeutic reel Mishina [33]. Recent experiments were carried out by us, using similar methods Kholmanskiy & Smirnov [16]; Kholmanskiy [18]. The results and experimental conditions are shown in Table 3. The errors in measuring the pigment content in all samples were about 10%. From the data of Table 3 it follows that the influence of EMF affects the efficiency of pigment biosynthesis only in a tree. The reason for this may be the elongation of transport communications along which charged mineral elements move from bottom to top, and phytohormones from top to bottom. The electrophysical properties of the sap in the layers of cambium and sapwood of deciduous trees, in contrast to the resin of conifers, contribute to electro tropism Kholmanskiy [12].

Conclusion

Thus, it was found that the content of chlorophyll and magnesium in the leaves of cultivated plants is significantly higher in fruitful species than in leafy and, especially in steppe grasses. The chlorophyll content increases when growing plants in light regime using LED irradiators having a radiation band that overlaps well with the first absorption band of chlorophyll a and b. Differences in the content of chlorophyll and plant productivity are associated with a more complex genetic program of metabolism in higher cultivated plants, including the stages of flowering and fruiting.

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Monday, January 18, 2021

Periodic Limb Movements in Sleep (PLMS) and Restless Leg Syndrome (RLS) - Juniper Publishers

 Orthopedic & Orthoplastic Surgery - Juniper Publishers

Abstract

Repetitive leg movements in sleep and Restless Leg Syndrome as two pathologies which can affect various patient groups like ones with neurologic, orthopedic, rheumatic diseases etc. and should be diagnosed and treated properly. There are some signs and symptoms which are related to these pathologies which can help the health care professionals to diagnose these pathologies properly. Also finding the best treatment strategies for these pathologies is of importance.

Keywords: Periodic Limb Movements in Sleep; Restless Leg Syndrome

Abbreviations: PLMS: Periodic Limb Movements in Sleep; RLS: Restless Leg Syndrome

Introduction

Repetitive leg movements in sleep and Restless Leg Syndrome are two pathologies which can be seen in various patient groups including ones with neurologic, orthopedic, rheumatic diseases etc. This is a brief review on these pathologies [1-2].

Discussion

Repetitive leg movements in sleep which last about 20 to 40 seconds and occur hundreds of time which cause awakenings during the sleep and consequently fragmented sleep, are the characteristics of the Periodic Limb Movements in Sleep or PLMS [3-4]. Insomnia can be seen in the patients with PLMS either. Periodic Limb Movement index, which is defined by the limb movements number in an hour of sleep, can be used for PLMS diagnosis. Leg movement study during the night with ten or more movements during an hour, can be used for clinical diagnosis of the PLMS. PLMS can be seen in the patients with narcolepsy, sleep-disordered breathing and fibromyalgia [5-6]. PLMS prevalence in adults is about 5 to 6 percent, although this rate would increase with age in a dramatic manner. For example, this rate can be increased to 45 percent in the patients with more than 65 years of age.

Leg dysesthesia or a creepy crawling or restless sensation which occur in a relaxed and awaked state and will be relieved by limb movement, is a characteristic of a syndrome which is called Restless Leg Syndrome (RLS) which is strongly related to the PLMS. Restless Leg Syndrome can be diagnosed by taking history from the patient or his/her bed partner by questioning about having restless and unpleasant feelings in their legs during relaxation or sleeping in the evening or at night which would be relieved by limb movement or walking. Since peripheral neuropathy, anemia or uremia can cause or exacerbate the restless leg syndrome, patients with RLS should be evaluated for having these concomitant problems either [7-9]. Dopamine agonists can be used to treat PLMS and RLS. Pramipexole and Ropinirole are the medications which are approved to treat Restless Leg Syndrome.

Conclusion

It is important for the health care professionals to have enough knowledge about PLMS and RLS, to treat the affected patients better during clinical practice.

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