Showing posts with label Spices. Show all posts
Showing posts with label Spices. Show all posts

Thursday, June 27, 2024

Spices in Indonesian History and Its Revival - Juniper Publishers

Archaeology & Anthropology - Juniper Publishers

Abstract

This article discusses the fluctuating importance of spices in history with a particular reference to Indonesia. Drawing upon secondary historical materials, it shows that spices once played a central role as the driving force of world history. They became a source of prosperity in many parts of the world where the commodities flowed to reach their final destinations. The quest for spices had created fierce competition among the Western nations for spices monopoly, which led to the development of Western colonialism and imperialism. From the most needed products, spices gradually lost their importance and became marginal in the colonial economy. New commodities such as coffee, sugar, tobacco, and rubber developed and became important under the Western colonial systems. After going unnoticed for about two centuries, new interests in spices are returning, thanks to the growing public and academic interest in spices, which the Covid-19 pandemic has also accelerated.

Keywords:Spices; Indonesian History; Fluctuating Importance; Revival

Introduction

Spices are essential commodities that have influenced the development of world history for centuries. World demand for spices grew throughout the Roman era and into the medieval period. The demand for spices has influenced economic development from India to Europe. This demand gave birth to ancient international trading networks that shaped the structure of the world economy in a way and influence that can still be felt today. Mastery over spices proved capable of enlivening many regions and diverting the flow of wealth worldwide. The story of spices has become an essential part of many nations’ historical development. It is common knowledge that spices are the main reason for exploration by Western nations in search of a new world.

The factor that determined the start of the story of the exploration of the world by the Portuguese and European nations was the control of Constantinople by the Turks, who then expanded to Asia Minor and Greece. The domination of the Turkish Islamic Empire in the Middle East had threatened the spice trade to Europe due to the protracted tensions between the Islamic world and the Western Christian world. In the era of ancient trade, products from Asia were on their way to Europe through three main gates, namely the Malacca Strait, the Persian Gulf, and the Red Sea. The emergence of Turkish power has blocked the trade flow of Asian goods to the West. In 1498, Portuguese explorer Vasco da Gama made the first sea voyage from Europe to India, skirting the west coast and the southernmost tip of the African continent [1]. Its most important mission was to find direct routes to places where spices were abundant at low prices without going through very long intermediary traders from producers in the Archipelago to consumers in Europe. This article elaborates the fluctuating importance of spices and its revival in the recent period after losing its important role due to the development of new commercial commodities under the colonial rule.

Materials and Methods

This article used secondary source materials in the form of historical books and relevant articles. Among the used sources are classic books on spices and their trade in the Indonesian Archipelago in the past that have been produced. It employed a historical method that generally includes four main stages: heuristics, source criticism, interpretation, and historiography [2,3]. The information taken from the collected sources is critically treated to establish facts that are considered credible. The collected facts were then interpreted and composed into a historical argument of the article.

The Quest for Spices

The arrival of Da Gama on the Malabar Coast of India, the heart of the spice trade, marked the start of an ambition to dominate the trade in these exotic products, which involved a chain link between Europe and Southeast Asia. Portuguese control of the spice route is regarded as a heavy blow to traders in the Arab world and the cities of the Mediterranean Sea [4]. The desire to establish direct trade could gradually be realized after the Portuguese were able to control important cities such as Goa in India (1510), the City of Malacca (1511), the City of Ormuz in the Persian Gulf (1514) [1]. The shifting of spice transportation routes made intermediary traders lose their role in the spice trade chain that connected Asia and Europe. The trading cities of the Arab world and Egypt suffered a setback. They languished because of the disappearance of the spices trade in their cities, which had been a source of livelihood and prosperity for centuries. Islamic traders and trading cities in the Mediterranean world were victims of the revolution in the spice trade route introduced by the Portuguese [1].

Spices were an excellent commodity that was contested. The desire to obtain monopoly rights over the spice trade has created competition, often leading to conflicts and wars between imperialist powers. The four giant European countries, Spain, Portugal, England, and the Netherlands, were involved in fierce competition for control of spices. These four European countries mobilized all their resources from the capital, knowledge of sea navigation, ship technology, war equipment, logistical supplies, and cartography, to skilled sailors, on long journeys for spices [5].

The quest for spices had sucked up a lot of energy and resources, ranging from enormous costs for years of exploration, wars between countries, and subjugation of local people who refuse the forced control of spices. The spice commodity invited the Dutch to the Archipelago, who later formed a trade partnership called Vereenigde Oostindische Compagnie (VOC). By force of arms, the VOC tried to impose a monopoly on the spice trade against various indigenous power centers in the Archipelago that controlled the production and trade of spices [5]. Through military conquest, the VOC successively obtained pepper monopoly rights in Palembang in 1642 [6], followed by the capture of the monopoly on the spice trade from the Sultanate of Makassar in 1669, and from the Sultanate of Banten in 1682. Around 1700, the VOC already held trade monopolies in Maluku, Makassar, Banten, Jambi and various other places [1].

Why were spices so tempting to European nations that they did not hesitate to shed blood to master them? For European nations, spices have many benefits. First, spices were essential in the taste of Western nations. The use of spices became a critical element of the European diet as a food flavoring ingredient which became a show of prestige at the dinner table among European aristocrats. Spices also presented many nuances of exotica for those who served and consumed them. However, the taste was only the reason for some things. Many reasons still strengthened spices’ charm and appeal [7].

The uses of spices were very diverse. Spices are used as food preservatives, medicines, burial processes, room deodorizers, and cult paraphernalia and are even used as sexual stimulants [7]. The use of vibrant spices has made the value of spices surpass precious metals in the past. The extraordinary benefits of this spice have encouraged the search for spices in the “new world” by Europeans since the fifteenth century, which was first carried out by Christophorus Columbus, an Italian explorer employed by the Spanish Empire in 1492, but a Portuguese explorer, Vasco Da Gama was more often regarded as a pioneer of the spice route, which began exploring the paradise of spices since 1497 [4,7].

Declining Role of Spices and New Commodities

The heyday of spices has indeed receded into the background, especially since the nineteenth century. Its prestige was fading, and its role as an export commodity for world markets was shrinking due to being eroded by new commodities that have sprung up and dominated trade in international markets. Since the collapse of the VOC trading partnership, Dutch interests in Indonesia began to evolve from a nation of traders who originally played a limited role as collectors of Indonesian products to a colonial nation with ambitions to build colonies and intervene in the process of plant cultivation. This laid the foundations through the control of the territory and the formation of the Dutch East Indies colonial state. Coffee, sugar, and other commodities, especially Java, became essential products produced in the Dutch East Indies. The growing global demand for plantation products has driven the large-scale conversion of forests to land for coffee plantations. Likewise, established rice fields and rural peasants were integrated into the sugar commodity production system to serve the interests of the colonial government in colonial exploitation ambitions to make the most of the opportunities arising from growing global market demand [8].

During the 19th and 20th centuries, spices pushed aside their prestige. The role of spices has become increasingly nebulous, pushed back and far from the center of attention with the advent of the tobacco commodity in the second half of the 19th century and rubber since the early 20th century. The role of spices as the main force driving historical developments has been replaced by plantation commodities such as coffee, sugar, rubber, and palm oil. The heyday of the Dutch East Indies colonial power was then more often identified with the exploitation of plantations, which for more than a century had gripped and transformed the society and economy of colonial Indonesia.

Under the government-sponsored Cultivation System and later through the commercial plantation system run by European companies, Indonesia’s territory has shifted its image from a country of spices to a country producing coffee, sugar, tobacco, and rubber [8]. This legacy continued into the independence era during the Sukarno and Suharto eras, making plantations a mainstay sector to support the national economy. During the New Order era, history witnessed how oil palm plantations experienced rapid expansion outside Java, especially Sumatra, and Kalimantan. The glory of spices and the prosperity that resulted from them have faded from memory.

Reviving Spices

Spices that have long been forgotten have again attracted widespread attention, and their revitalization was fought for in the early 21st century through public literacy. At least two critical factors are driving this development. First, the success of China in making part of the silk route in its territory a UNESCOrecognized world heritage. The Chinese Section of the Silk Road includes the Land Route and the Sea Route. This Overland Route covered ancient trade routes that started in ancient Chang’an, the city of present-day Xi’an, and the center of politics, economy, and culture in the old period of ancient China. This route refers to the commercial overland route connecting Asia. Africa and Europe also serve as main avenues of economic, political, and cultural exchange between East and West. At first, the function of trade routes was to transport silk, an elegant and expensive product that represented ancient Chinese civilization. Meanwhile, the Silk Road Seaway was a maritime trade route connecting East and West, using monsoons, ocean currents, and traditional navigation technologies [9]. The Chinese section of the Silk Road can be seen as a cultural bridge connecting different regions and countries with rich historical information. Therefore, when the name “Silk Road” was first given by the German geographer Ferdinand Freiherr von Richthofen in the 1870s, this name became widely accepted [9].

The success of China’s cultural politics has inspired and aroused intellectual interest in Indonesia to revive Indonesia’s historical role as a spice haven and for this role to gain formal international recognition. Various events/activities were held to echo Indonesia’s significant role in the global spice route, including the “Spice Route: Untold Story” exhibition held at the National Museum in Jakarta on 18-25 October 2015; The Nusantara Spice Trail Exhibition, which was held on 4-18 November 2017, the 2018 Spice Route Expedition, The History of the Spice Trail and Kie Raha’s Biological Wealth, which was held by the Ministry of Education and Culture 28 September-10 October 2018 [10].

The precedent for a revival of attention and interest in spices is inseparable from the publications about spices that have appeared since the 2000s. Several essential publications can be mentioned here; among them is the work by Robin A. Donkin, Between East and West: the Moluccas and the Traffic in Spices up to the Arrival of Europeans, which highlights the traffic of the spice trade from Maluku that links the East and the West [11], followed by the publication of The History of a Temptation (2004) by Jack Turner [7] and Out of the East: Spices and the Medieval Imagination (2008) by Paul Freedman [12]. Fred Czarra’s work (2009), Spices: A Global History, examines spices from a global historical perspective [13]. It is also necessary to mention the publication of M. Adnan Amal (2016), The History of the Spice Islands: The Historical Journey of North Maluku, which reviews the long journey of about seven centuries of North Maluku history as a producer of spices [14,10].

The second factor that is no less important is the Covid-19 pandemic. The global panic caused by this outbreak has stimulated interest in utilizing the wealth of spices as an alternative weapon to maintain the body’s immunity so that it is not easily infected with the virus that has shaken people worldwide. The passion for consuming spices and herbal ingredients is increasing among people. Many people searched for all kinds of spices in the markets, so prices soared, and it was common for the items sought to become rare and difficult to obtain because many consumers needed them. No less excited, in the academic world, spices have become a hot topic and are widely discussed in academic forums. Among the various events that were held, for example, a discussion with the theme “Spices in Commerce and Medicine” was held by BPNB Yogyakarta on June 17, 2020; The seminar “Path Versus Network: Spices Warm the World” was held by the Directorate General of Culture, Ministry of Education and Culture on July 24, 2020. The National Archives of the Republic of Indonesia also held a series of seminars on the spice route, for example, ‘Preservation and Restoration of Ancient Manuscripts and Archival Sites/ Artifacts Spice Path’ on November 26, 2020 and “Tracing the Spice Trails Through Archives, Ancient Manuscripts, Sites and Artifacts” December 4, 2020. There are still many similar events to be added to the list, but all of them indicate one thing: the growth of new hopes for the wealth of spices as a valuable capital to build the nation’s prosperity.

Conclusion

Historical experience in the past clearly shows that the story of spices has linked the Western and Eastern worlds, forming a global network that involves many nations, bringing together various ideas and influences in economic, sociological, political, and even cultural dimensions. Revitalizing spices requires crossdisciplinary collaborative efforts. The diversity of perspectives does not need to be contested but synergized as collective capital and strength in revitalizing spices. The revitalization of spices is not only a matter of technical cultivation but also related to more complex and broader aspects. An essential part of it is awareness and the ability to appreciate the richness of spices in cultural spaces creatively. In this context, spice literature in the large family of spice humanities has the potential to make a real and actual contribution to human civilization.

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Thursday, May 2, 2024

Characterization of Aroma Active Compounds of Cumin (Cuminum cyminum L.) Seed Essential Oil - Juniper Publishers

 Bioequivalence & Bioavailability - Juniper Publishers

Abstract

Cumin (Cuminum cyminum L.) is one such most popular spice that is used as a culinary spice for their special aromatic effect. The flavor of cumin is judged by its volatile oil content. The advantage of use of volatile oil is that it is 100 times more concentrated then the spice powder and hence is required in a very less quantity. The essential oil is responsible for the characteristic cumin odor. In present study evaluation of fragrance and flavor profile in essential oil of cumin from the Algerian market (Algeria, Northwest Africa) has been identified. The essential oil from the seeds of Cuminum cyminum L. was isolated by hydro-distillation method and the chemical composition was determined by gas chromatography-mass spectrometry. Eighteen (18) components representing (91.10%) of the essential oil were identified. β-pinene (9.5%), γ-terpinene (10.0%), p-cymene (11.8%) and Cuminaldehyde (50.5%) were the major components. The essential oil was also subjected to measurement of the physicochemical properties; refractive index (20 °C): 1.48, density (20 °C): 0.91, alcohol solubility (80% v/v): 1.1, aldehyde percentage: 50%, acidity: 1.0, alcohol percentage: 3.5%, carbonyl index: 9.32 and steric index: 19.24. These results suggested that the Cuminum cyminum L. essential oil is a potential source of active ingredients for food, pharmaceutical and cosmetic industry.

Keywords: Spices; Cumin; Cuminum cyminum L.; Essential oil; GC-MS; Physicochemical properties

Abbreviations: GC-MS: Gas Chromatography-Mass Spectroscopy; MSD: Mass Selective Detector; ISO: International Organization for Standardization; French AFNOR: French Association of Normalization

Introduction

Since earliest times medicinal plants have played a vital role in the development and comfort of human civilization. Many of the plants have medicinal properties that reduce symptoms or prevent diseases [1]. Spices are widely used in the Mediterranean countries of North Africa and Southern Europe. They are also used for their flavors and aromas and for the sensations they produce. They can also be used as food colorants and antioxidants [2].

Originally from the Mediterranean area [3], Cuminum cyminum L. is an annual herbaceous plant which grows up to 15-50cm height somewhat angular and tends to droop under its own weight. It has a long, white root. The leaves are 5-10cm long, pinnate or bi pinnate, with thread-like leaflets and blue green in color and are finely divided, generally turned back at the ends. The leaves are highly dissected. Whitish-red flowers are on a compound umbel (arrangement of flowers looks like an umbrella). The fruit is an elongated, oval shaped schizocarp (an aggregate fruiting body which doesn’t break open naturally and has two single seeded units called mericarps). The fruits are similar to fennel seeds, when chewed has bitter and pungent taste. The fruit are thicker in the middle, compressed laterally about 5 inch-long, containing a single seed [4].

Although the seeds of cumin (Cuminum cyminum L.) are widely used as a spice for their distinctive aroma, they are also commonly used in traditional medicine to treat a variety of diseases. The literature presents ample evidence for the biomedical activities of cumin, which have generally been ascribed to its bioactive constituents such as terpenes, phenols, and flavonoids. Multiple studies made in the last decades validate its health beneficial effects particularly in diabetes, dyslipidemia, hypertension, respiratory disorders, inflammatory diseases, and cancer. Cumin seeds are nutritionally rich; they provide high amounts of fat (especially monounsaturated fat), protein, and dietary fiber. Vitamins B and E and several dietary minerals, especially iron, are also considerable in cumin seeds [5].

The Cumin oil is reported as a high antioxidant mainly due to the presence of monoterpene alcohols [6]. The presence of phytoestrogens in Cumin has been reported which related to its anti-osteoporotic effects. Methanol extract of Cumin showed a significant reduction in urinary calcium excretion and augmentation of calcium content and mechanical strength of bones in animals [7]. Furthermore, the aqueous extract of Cumin seeds indicated the protective effect against gentamycin-induced nephrotoxicity, which decreased the gentamycin-induced elevated levels of serum urea and enhanced the clearance of the drug [8].

Essential oils have become in recent years a matter of considerable economic importance, with a constantly growing market whose fields of application are directly related to human consumption. This is why essential oils are more and more controlled in order to verify the presence of certain toxic natural compounds, their natural origin or not, their source and the presence of certain compounds. active ingredients. The purpose of this study is to provide experimental data on the chemical composition and the physicochemical properties of cumin that could be considered suitable for application in foods and drugs.

Materials

Plant material and essential oil extraction

The seeds of the plant were used; the plant material was hydro- distilled for 90min using a Clevenger-type apparatus. (The extraction performed after a 4-hours maceration in 500ml of water). The essential oil obtained was then dehydrated over anhydrous sodium sulphate and stored in a refrigerator at 4 °C until use. The plant was identified by Dr. Hicham Boughendjioua at the Department of Natural Sciences, High School Professors Technological Education, Skikda (Algeria). The voucher specimen under the plant’s name deposited then in the herbarium.

GC-MS analysis

Gas chromatography-mass spectroscopy (GC-MS) analyses of essential oil samples were carried out on a Hewlett-Packard 6890N gas chromatograph coupled to a HP 5973 mass selective detector (MSD). A HP5 column (30m х 0.32mm film thickness 0.25μm) was used. The analysis was performed using the following temperature program: oven isotherm at 35 °C for 5 min then from 35 to 250 °C at 6 ºC/min. Helium was used as the carrier gas at 1ml/min flow rate. The injector and detector temperatures were held, respectively, at 250 ºC. Mass spectra were recorded with ionization energy of 70eV and interface temperature of 280 °C. The identification of the oil constituents was based on a comparison of their retention indices relative. Further identification was made by matching their recorded mass spectra with those stored in the NIST mass spectral library of the GC-MS data system.

Results and Discussion

Classification of cumin

The plant was classified according to APG system III, 2009 (Table 1) [9].

Modern Applications of Bioequivalence & Bioavailability

Essential oil yield

The extracted cumin essential oil has dark yellow color, with an odor hot, powerful and spicy. The percentage yield of essential oil was calculated as per Moawad et al. [10], it is calculated on the weight basis. The equation is as follows: Volatile oil (%) = (Weight of the volatile essential oil recovered in g x 100)/Weight of sample taken in g. Yield estimation studies indicate that the value of essential oil was: 3.66%.

Physicochemical properties

Essential oils must meet characteristics imposed by the laws of producing and exporting countries and by importing countries. These criteria are defined in international standards ISO (International Organization for Standardization) or French AFNOR (French Association of Normalization). Thus, the organoleptic and physical properties such as coloration, odor, refraction, solubility, flash point, but also chemical properties such as acid and ester indices are controlled [11]. Physicochemical properties of the essential oil obtained by hydro-distillation from Cumin seeds are summarized in Table 2.

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Chemical composition

Due to the enormous amount of raw product used to make wholly natural essential oils, it is important to study the chemical composition of the volatile fraction once the essential oil is extracted. Essential oils are hydrophobic and concentrated liquids whose composition is complex. The best qualitative and quantitative identity card of an essential oil, however, remains its chromatographic profile, most of which is carried out in gas chromatography.

The chemical compositions of Cuminum cyminum L. essential oil are shown in Table 3, Figure 1. Eighteen (18) components representing 91.10% of the essential oil were identified. β-pinene (9.5%), γ-terpinene (10.0%), p-cymene (11.8%) and Cuminaldehyde (50.5%) were the major components.

Modern Applications of Bioequivalence & Bioavailability
Modern Applications of Bioequivalence & Bioavailability

The essential oil of the seeds of Cuminum cyminum L. from China was isolated by hydrodistillation in a yield of 3.8%. The chemical composition of the essential oil was examined by GC and GC-MS; 37 components, representing 97.97% of the oil, were identified. Cuminal (36.31%), cuminic alcohol (16.92%), γ-terpinene (11.14%), safranal (10.87%), p-cymene (9.85%) and β-pinene (7.75%) were the major components [12].

The main constituents at different harvesting time being cumin aldehyde (19.9-23.6%), p-mentha-1,3-dien-7-al (11.4-17.5%) and p-mentha-1,4-dien-7-al (13.9-16.9%). The results of GC and GC/MS analysis showed that the fruits should be harvested at the ripe stage for ideal volatile oil yield and composition [13].

GC and GC-MS analyses of the essential oil of Cuminum cyminum L. from the Alborz Mountain range of Iran revealed contained α-pinene (29.2%), limonene (21.7%), 1,8-cineole (18.1%), linalool (10.5%), and α-terpineole (3.17%) as the major compounds [14].

Cuminum cyminum L. seeds essential oil was isolated by hydrodistillation method and the chemical composition was determined by gas chromatography-mass spectrometry (GC/MS). The yield of the oil was found to be 3.0% (on dry weight basis). A total of twenty-six components, representing 96.7% of the oil were identified. Cuminaldehyde (49.4%), p-cymene (17.4%), β-pinene (6.3%), α-terpinen-7-al (6.8%), γ- terpinene (6.1%), p-cymen-7- ol (4.6%) and thymol (2.8%) were the major components in the oil [15].

Composition of the essential oil, which was obtained from the seeds of Cuminum cyminum L. collected from Ilam, was determined by GC-MS. In total, 25 components (83.36%) of essential oil were identified. Major constituents were Isobutyl isobutyrate (0.45%), α-thujene (0.5%), α-pinene (30.12%), sabinene (1.11%), myrcene (0.34%), γ-3-carene (0.21%), p-cymene (0.6%), limonene (10.11%), 1,8-cineole (11.54%), (E)-ocimene (0.1%), γ-terpinene (3.56%), terpinolene (0.32%), linalool (10.3%), α-campholenal (1.76%), terpinene-4-ol (0.6%), trans-carveole (0.7%), geraniol (1.0%), linalyl acetate (4.76%), α-terpinyl acetate (1.8%), neryl acetate (1%), methyl eugenol (0.2%), β-caryophyllene (0.42%), α-humulene (0.3%), spathulenol (0.56%) and humulene epoxide II (1%) [16].

The essential oil content in cumin samples from Serbian market ranged between 2.0 and 4.0%, with 22 identified compounds, among which the most abundant were cumin aldehyde, β-pinene, γ-terpinene, γ-terpinene-7 al and p-cymene. Post-distillation cumin seeds waste material that remained after the essential oil extraction contains total polyphenols of between 30.1 and 47.5 mg GAE/g dry extract, as estimated by the Folin Ciocalteu method. Hydroxybenzoic and hydroxycinnamic acids, as well as glycosides of flavonones and flavonoles, are the dominant polyphenols [17].

The major constituents of the essential oil from the cumin fruits under different conditions of storage were cumin aldehyde belonging to oxygenated monoterpenes and p-cymene, and β-pinene belonging to monoterpene hydrocarbons. Results indicated that at room temperature, the proportions of compounds with lower boiling temperatures such as β-pinene (1.57-10.03%) and p-cymene (14.93-24.9%) were decreased; however, cumin aldehyde (45.45-64.31%) increased during cumin oil storage [18].

The GC-MS analysis of cumin oil showed that eleven constituents were identified; seven hydrocarbon monoterpens (33.09%) and four oxygenated monoterpens (66.92%). The monoterpens were α-thujene (0.41%), α-pinene (0.90%), β-pinene (10.72%), β-myrcene (1.27%), α-phellandrene (1.18%), p-cymene (3.54%) and γ-terpinene (15.07%), and oxygenated monoterpens identified were cumin aldehyde (21.10%), carboxaldehyde (5.34%), 2-caren-10-al (17.74%) and cumin alcohol (22.65%) [19].

This deviation from the common chemo-types may be attributed to the effect of the factors that specifically affect the composition and yield of the essential oil, which include seasonal and maturity variation, geographical origin, genetic variation, growth stages, postharvest drying and storage [20-23].

Conclusion

Cumin (Cuminum cyminum L.) is the second most popular spice in the world, after black pepper, and used as a medicinal plant for aromatherapy and various illnesses. Determination of the physicochemical characteristics of the oil may establish by measurement of extraction yield, refractive index, density, carbonyl and steric indexes together with aldehyde, alcohol and acid contents.

In the chemical profiling, eighteen (18) components representing (91.10%) of the essential oil were identified, of which Cuminaldehyde with a concentration of (50.5%) was the main constituent, the physicochemical properties of the essential oil were also subjected to study (measurement).

Essential oils have become in recent years a matter of considerable economic importance, with a constantly growing market whose fields of application are directly related to human consumption. This is why essential oils are more and more controlled in order to verify the presence of certain natural toxic compounds, their natural or non-natural origin, their source and the presence of certain active compounds and even though the plant biomass a very promising source for the future, very little works has been done on the study of the organoleptic and physicochemical properties of aromatic fractions of cumin. Due to its chromatographic profile, the essential oil extracted by hydrodistillation of this plant has organoleptic and physicochemical properties very appreciated in perfumery and will be very coveted in the sector of the food, pharmaceutical and cosmetic industry.

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Friday, December 4, 2020

Characterization of Aroma Active Compounds of Cumin (Cuminum cyminum L.) Seed Essential Oil - Juniper Publishers

 Bioequivalence & Bioavailability - Juniper Publishers   


Abstract

Cumin (Cuminum cyminum L.) is one such most popular spice that is used as a culinary spice for their special aromatic effect. The flavor of cumin is judged by its volatile oil content. The advantage of use of volatile oil is that it is 100 times more concentrated then the spice powder and hence is required in a very less quantity. The essential oil is responsible for the characteristic cumin odor. In present study evaluation of fragrance and flavor profile in essential oil of cumin from the Algerian market (Algeria, Northwest Africa) has been identified. The essential oil from the seeds of Cuminum cyminum L. was isolated by hydro-distillation method and the chemical composition was determined by gas chromatography-mass spectrometry. Eighteen (18) components representing (91.10%) of the essential oil were identified. β-pinene (9.5%), γ-terpinene (10.0%), p-cymene (11.8%) and Cuminaldehyde (50.5%) were the major components. The essential oil was also subjected to measurement of the physicochemical properties; refractive index (20 °C): 1.48, density (20 °C): 0.91, alcohol solubility (80% v/v): 1.1, aldehyde percentage: 50%, acidity: 1.0, alcohol percentage: 3.5%, carbonyl index: 9.32 and steric index: 19.24. These results suggested that the Cuminum cyminum L. essential oil is a potential source of active ingredients for food, pharmaceutical and cosmetic industry.

Keywords: Spices; Cumin; Cuminum cyminum L.; Essential oil; GC-MS; Physicochemical properties

Abbreviations: GC-MS: Gas Chromatography-Mass Spectroscopy; MSD: Mass Selective Detector; ISO: International Organization for Standardization; French AFNOR: French Association of Normalization

Introduction

Since earliest times medicinal plants have played a vital role in the development and comfort of human civilization. Many of the plants have medicinal properties that reduce symptoms or prevent diseases [1]. Spices are widely used in the Mediterranean countries of North Africa and Southern Europe. They are also used for their flavors and aromas and for the sensations they produce. They can also be used as food colorants and antioxidants [2].

Originally from the Mediterranean area [3], Cuminum cyminum L. is an annual herbaceous plant which grows up to 15-50cm height somewhat angular and tends to droop under its own weight. It has a long, white root. The leaves are 5-10cm long, pinnate or bi pinnate, with thread-like leaflets and blue green in color and are finely divided, generally turned back at the ends. The leaves are highly dissected. Whitish-red flowers are on a compound umbel (arrangement of flowers looks like an umbrella). The fruit is an elongated, oval shaped schizocarp (an aggregate fruiting body which doesn’t break open naturally and has two single seeded units called mericarps). The fruits are similar to fennel seeds, when chewed has bitter and pungent taste. The fruit are thicker in the middle, compressed laterally about 5 inch-long, containing a single seed [4].

Although the seeds of cumin (Cuminum cyminum L.) are widely used as a spice for their distinctive aroma, they are also commonly used in traditional medicine to treat a variety of diseases. The literature presents ample evidence for the biomedical activities of cumin, which have generally been ascribed to its bioactive constituents such as terpenes, phenols, and flavonoids. Multiple studies made in the last decades validate its health beneficial effects particularly in diabetes, dyslipidemia, hypertension, respiratory disorders, inflammatory diseases, and cancer. Cumin seeds are nutritionally rich; they provide high amounts of fat (especially monounsaturated fat), protein, and dietary fiber. Vitamins B and E and several dietary minerals, especially iron, are also considerable in cumin seeds [5].

The Cumin oil is reported as a high antioxidant mainly due to the presence of monoterpene alcohols [6]. The presence of phytoestrogens in Cumin has been reported which related to its anti-osteoporotic effects. Methanol extract of Cumin showed a significant reduction in urinary calcium excretion and augmentation of calcium content and mechanical strength of bones in animals [7]. Furthermore, the aqueous extract of Cumin seeds indicated the protective effect against gentamycin-induced nephrotoxicity, which decreased the gentamycin-induced elevated levels of serum urea and enhanced the clearance of the drug [8].

Essential oils have become in recent years a matter of considerable economic importance, with a constantly growing market whose fields of application are directly related to human consumption. This is why essential oils are more and more controlled in order to verify the presence of certain toxic natural compounds, their natural origin or not, their source and the presence of certain compounds. active ingredients. The purpose of this study is to provide experimental data on the chemical composition and the physicochemical properties of cumin that could be considered suitable for application in foods and drugs.

Materials

Plant material and essential oil extraction

The seeds of the plant were used; the plant material was hydro- distilled for 90min using a Clevenger-type apparatus. (The extraction performed after a 4-hours maceration in 500ml of water). The essential oil obtained was then dehydrated over anhydrous sodium sulphate and stored in a refrigerator at 4 °C until use. The plant was identified by Dr. Hicham Boughendjioua at the Department of Natural Sciences, High School Professors Technological Education, Skikda (Algeria). The voucher specimen under the plant’s name deposited then in the herbarium.

GC-MS analysis

Gas chromatography-mass spectroscopy (GC-MS) analyses of essential oil samples were carried out on a Hewlett-Packard 6890N gas chromatograph coupled to a HP 5973 mass selective detector (MSD). A HP5 column (30m х 0.32mm film thickness 0.25μm) was used. The analysis was performed using the following temperature program: oven isotherm at 35 °C for 5 min then from 35 to 250 °C at 6 ºC/min. Helium was used as the carrier gas at 1ml/min flow rate. The injector and detector temperatures were held, respectively, at 250 ºC. Mass spectra were recorded with ionization energy of 70eV and interface temperature of 280 °C. The identification of the oil constituents was based on a comparison of their retention indices relative. Further identification was made by matching their recorded mass spectra with those stored in the NIST mass spectral library of the GC-MS data system.

Results and Discussion

Classification of cumin

The plant was classified according to APG system III, 2009 (Table 1) [9].

Modern Applications of Bioequivalence & Bioavailability

Essential oil yield

The extracted cumin essential oil has dark yellow color, with an odor hot, powerful and spicy. The percentage yield of essential oil was calculated as per Moawad et al. [10], it is calculated on the weight basis. The equation is as follows: Volatile oil (%) = (Weight of the volatile essential oil recovered in g x 100)/Weight of sample taken in g. Yield estimation studies indicate that the value of essential oil was: 3.66%.

Physicochemical properties

Essential oils must meet characteristics imposed by the laws of producing and exporting countries and by importing countries. These criteria are defined in international standards ISO (International Organization for Standardization) or French AFNOR (French Association of Normalization). Thus, the organoleptic and physical properties such as coloration, odor, refraction, solubility, flash point, but also chemical properties such as acid and ester indices are controlled [11]. Physicochemical properties of the essential oil obtained by hydro-distillation from Cumin seeds are summarized in Table 2.

Modern Applications of Bioequivalence & Bioavailability

Chemical composition

Due to the enormous amount of raw product used to make wholly natural essential oils, it is important to study the chemical composition of the volatile fraction once the essential oil is extracted. Essential oils are hydrophobic and concentrated liquids whose composition is complex. The best qualitative and quantitative identity card of an essential oil, however, remains its chromatographic profile, most of which is carried out in gas chromatography.

The chemical compositions of Cuminum cyminum L. essential oil are shown in Table 3, Figure 1. Eighteen (18) components representing 91.10% of the essential oil were identified. β-pinene (9.5%), γ-terpinene (10.0%), p-cymene (11.8%) and Cuminaldehyde (50.5%) were the major components.

Modern Applications of Bioequivalence & Bioavailability
Modern Applications of Bioequivalence & Bioavailability

The essential oil of the seeds of Cuminum cyminum L. from China was isolated by hydrodistillation in a yield of 3.8%. The chemical composition of the essential oil was examined by GC and GC-MS; 37 components, representing 97.97% of the oil, were identified. Cuminal (36.31%), cuminic alcohol (16.92%), γ-terpinene (11.14%), safranal (10.87%), p-cymene (9.85%) and β-pinene (7.75%) were the major components [12].

The main constituents at different harvesting time being cumin aldehyde (19.9-23.6%), p-mentha-1,3-dien-7-al (11.4-17.5%) and p-mentha-1,4-dien-7-al (13.9-16.9%). The results of GC and GC/MS analysis showed that the fruits should be harvested at the ripe stage for ideal volatile oil yield and composition [13].

GC and GC-MS analyses of the essential oil of Cuminum cyminum L. from the Alborz Mountain range of Iran revealed contained α-pinene (29.2%), limonene (21.7%), 1,8-cineole (18.1%), linalool (10.5%), and α-terpineole (3.17%) as the major compounds [14].

Cuminum cyminum L. seeds essential oil was isolated by hydrodistillation method and the chemical composition was determined by gas chromatography-mass spectrometry (GC/MS). The yield of the oil was found to be 3.0% (on dry weight basis). A total of twenty-six components, representing 96.7% of the oil were identified. Cuminaldehyde (49.4%), p-cymene (17.4%), β-pinene (6.3%), α-terpinen-7-al (6.8%), γ- terpinene (6.1%), p-cymen-7- ol (4.6%) and thymol (2.8%) were the major components in the oil [15].

Composition of the essential oil, which was obtained from the seeds of Cuminum cyminum L. collected from Ilam, was determined by GC-MS. In total, 25 components (83.36%) of essential oil were identified. Major constituents were Isobutyl isobutyrate (0.45%), α-thujene (0.5%), α-pinene (30.12%), sabinene (1.11%), myrcene (0.34%), γ-3-carene (0.21%), p-cymene (0.6%), limonene (10.11%), 1,8-cineole (11.54%), (E)-ocimene (0.1%), γ-terpinene (3.56%), terpinolene (0.32%), linalool (10.3%), α-campholenal (1.76%), terpinene-4-ol (0.6%), trans-carveole (0.7%), geraniol (1.0%), linalyl acetate (4.76%), α-terpinyl acetate (1.8%), neryl acetate (1%), methyl eugenol (0.2%), β-caryophyllene (0.42%), α-humulene (0.3%), spathulenol (0.56%) and humulene epoxide II (1%) [16].

The essential oil content in cumin samples from Serbian market ranged between 2.0 and 4.0%, with 22 identified compounds, among which the most abundant were cumin aldehyde, β-pinene, γ-terpinene, γ-terpinene-7 al and p-cymene. Post-distillation cumin seeds waste material that remained after the essential oil extraction contains total polyphenols of between 30.1 and 47.5 mg GAE/g dry extract, as estimated by the Folin Ciocalteu method. Hydroxybenzoic and hydroxycinnamic acids, as well as glycosides of flavonones and flavonoles, are the dominant polyphenols [17].

The major constituents of the essential oil from the cumin fruits under different conditions of storage were cumin aldehyde belonging to oxygenated monoterpenes and p-cymene, and β-pinene belonging to monoterpene hydrocarbons. Results indicated that at room temperature, the proportions of compounds with lower boiling temperatures such as β-pinene (1.57-10.03%) and p-cymene (14.93-24.9%) were decreased; however, cumin aldehyde (45.45-64.31%) increased during cumin oil storage [18].

The GC-MS analysis of cumin oil showed that eleven constituents were identified; seven hydrocarbon monoterpens (33.09%) and four oxygenated monoterpens (66.92%). The monoterpens were α-thujene (0.41%), α-pinene (0.90%), β-pinene (10.72%), β-myrcene (1.27%), α-phellandrene (1.18%), p-cymene (3.54%) and γ-terpinene (15.07%), and oxygenated monoterpens identified were cumin aldehyde (21.10%), carboxaldehyde (5.34%), 2-caren-10-al (17.74%) and cumin alcohol (22.65%) [19].

This deviation from the common chemo-types may be attributed to the effect of the factors that specifically affect the composition and yield of the essential oil, which include seasonal and maturity variation, geographical origin, genetic variation, growth stages, postharvest drying and storage [20-23].

Conclusion

Cumin (Cuminum cyminum L.) is the second most popular spice in the world, after black pepper, and used as a medicinal plant for aromatherapy and various illnesses. Determination of the physicochemical characteristics of the oil may establish by measurement of extraction yield, refractive index, density, carbonyl and steric indexes together with aldehyde, alcohol and acid contents.

In the chemical profiling, eighteen (18) components representing (91.10%) of the essential oil were identified, of which Cuminaldehyde with a concentration of (50.5%) was the main constituent, the physicochemical properties of the essential oil were also subjected to study (measurement).

Essential oils have become in recent years a matter of considerable economic importance, with a constantly growing market whose fields of application are directly related to human consumption. This is why essential oils are more and more controlled in order to verify the presence of certain natural toxic compounds, their natural or non-natural origin, their source and the presence of certain active compounds and even though the plant biomass a very promising source for the future, very little works has been done on the study of the organoleptic and physicochemical properties of aromatic fractions of cumin. Due to its chromatographic profile, the essential oil extracted by hydrodistillation of this plant has organoleptic and physicochemical properties very appreciated in perfumery and will be very coveted in the sector of the food, pharmaceutical and cosmetic industry.


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