Report on Isolation and Identification of Brucella Abortus from Aborted Foetus and Lymph Nodes of Beef Cattle in Malaysia (JDVS) - Juniper Publishers

Report on Isolation and Identification of Brucella Abortus from Aborted Foetus and Lymph Nodes of Beef Cattle in Malaysia in Juniper in Journal of Dairy & Veterinary Sciences

Isolation of living brucellae from tissues or organs such as aborted foetus and lymph nodes has traditionally provided the most accurate method for the detection of Brucella infection. The inoculated medium was incubated at 37 °C for 7 days with 10% CO2. Bacterial colonies growth on the agar was examined. The bacterial cells were stained with Gram-staining and Modified Ziehl-Neelsen staining methods and later were examined under microscope to determine their cell morphology. Biochemical tests were performed to complete the phenotypic and metabolic characterisation of the isolates. Brucella abortus were isolated from stomach content, lung and cotyledon of the aborted foetus; and supramammary and internal iliac lymph nodes of aborted cattle. Histopathological examination of lymph nodes found the infiltrations of microphages, neutrophils and giant cells with vacuolated and engorged macrophages scattered in the necrotic debris of supramamarry lymph node of aborted cattle.

https://juniperpublishers.com/jdvs/pdf/JDVS.MS.ID.555553.pdf

https://juniperpublishers.com/jdvs/JDVS.MS.ID.555553.php

Influence of Using a Blend of Rennet Casein and Whey Protein Concentrate as Protein Source on the Quality of Mozzarella Cheese Analogue (JDVS) - Juniper publishers

The Prolactin Gene Expression Variance in Marshes and Riverine Buffalos in Iraq by Dhia Hussain Jassim in Juniper in JDVS

The study area consisted of eight stations in southern Iraqi marshes in the Missan governorate, and six stations in the Al-Qadisiya and Al-Najaf governorates. The southern Iraqi marshes had the proper environment for the culture of the riverine buffalo breed, yet the water buffalo prefer the middle area [1], because the molecular information of the local buffalo in Iraq is very rare [2]. The present study selection is the improvement of milk yield with the advances in molecular biology, the identification of the underlying genes by quantitative (rt-PCR) technique responsible for the efficiency of the prolactin (PRL-R) gene expression and to better understand the actions of mammary gland gene expression on milk production in the two buffalo breeds in Iraq, by determining levels of the PRL-R gene expression in somatic cells in the mammary gland by random milk samples of swamp and riverine buffalos during first medal and last lactation period. The results showed high expression of PRL-R gene in last lactation period of riverine buffalo than in swamp buffalo breed, and hence this gene may have potential direct or indirect effect on milk production. The transcription levels of PRL-R gene in the milk of swamp buffalo were found to be significantly down regulated in the first stage of lactation period, but were up-regulated in second stage of this period, and this regulation significantly decreased in the last period. In the riverine buffalo, the levels were very down regulated in the first stage of and curved to a highly induced regulation in second and late lactation period. 

https://juniperpublishers.com/jdvs/pdf/JDVS.MS.ID.555551.pdf

https://juniperpublishers.com/jdvs/JDVS.MS.ID.555551.php