Validation of a Novel and Sensitive RP-HPLC Method for Simultaneous Determination of Cefixime Trihydrate and Sodium Benzoate in Powder for Oral Suspension Dosage Form by MEM Hassouna in JFSCI - Juniper Publishers
A novel, specific, precise, simple, and accurate RP-HPLC method is developed and validated for simultaneous determination of Cefixime trihydrate (CFX) and Sodium benzoate (SDM) in powder for oral suspension (POS) dosage form. RP-HPLC method is performed on the Agilent Eclipse XDB C8 column (250 mm X 4.6 mm, 5μm particle size, using buffer solution of pH 2.8 containing 0.02M sodium dihydrogen phosphate: Acetonitrile (50:50 v/v) as the mobile phase at a flow rate of 1.0 mL/min, injection volume 10 μL and UV detection at 229 nm. And the total run time was 7.0 min. Linear relationships are obtained in the ranges of 5-200 μg/mL and 2-40 μg/mL for CFX and SDM, respectively, with significantly different Rt values of 2.203 and 3.970 min for CFX and SDM with correlation coefficients (r) >0.9999, limits of detection of 1.36 and 0.21 μgmL-1 and limits of quantitation was 4.14 and 0.64 μgmL-1 for CFX and SDM, respectively.
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