Juniper Publishers

Juniper publishers have been established with the aim of spreading quality scientific information to the research community throughout the universe. We, as Open Access publishers, strive to offer the best in class online science publications. Open Access process eliminates the barriers associated with the older publication models, thus matching up with the rapidity of the twenty-first century. Our main areas of interest lie in the fields of science, engineering and other related areas.

Showing posts with label In Vitro. Show all posts

Monday, September 24, 2018

Crotalus lepidus lepidus Venom Produces Hemolysis and Lipid Peroxidation in Human Erythrocytes in vitro (OAJT) - Juniper Publishers

JUNIPER PUBLISHERS-OPEN ACCESS JOURNAL OF TOXICOLOGY

Crotalus lepidus lepidus Venom Produces Hemolysis and Lipid Peroxidation in Human Erythrocytes in vitro in Juniper in Open Access Journal of Toxicology

The hemolytic and lipid peroxidative effects of crude venom from Crotalus lepidus lepidus, a rattle snake found in the Northeast of Mexico and southeast of the United States, were investigated in human erythrocytes in vitro. Significant concentration-dependent effects were found on both hemolysis (evaluated as release of hemoglobin) and lipid peroxidation (as a common index of oxidative damage to membrane lipids) in the red blood cells. These results suggest that hemolysis was produced with the involvement of oxidative stress as a potential mechanism of toxicity in the erythrocytes.

https://juniperpublishers.com/oajt/pdf/OAJT.MS.ID.555554.pdf

https://juniperpublishers.com/oajt/OAJT.MS.ID.555554.php

Thursday, September 20, 2018

Rat Brain Acetyl Cholinesterase as a Biomarker of Cadmium Induced Neurotoxicity (OAJT) - Juniper Publishers

JUNIPER PUBLISHES-OPEN ACCESS JOURNAL OF TOXICOLOGY

Rat Brain Acetyl Cholinesterase as a Biomarker of Cadmium Induced Neurotoxicity in Juniper in Open Access Journal of Toxicology (OAJT)

Cadmium as potential environmental xenobiotics has been shown to cross blood brain barrier and to adversely influence the activity of AChE and hence the brain functions. In the present study, we have evaluated the impact of cadmium in vitro on the properties of AChE isolated from rat brain. The enzyme was found to be membrane bound and it could be successfully solubilized using 0.2% (v/v) Triton X-100, a nonionic detergent, in the extraction buffer (50mM Phosphate, pH 7.4). The enzyme was found to be highly stable up to one month when stored at -20°C. This enzyme exhibited maximum activity at pH 7.4. AChE when incubated at different temperatures for 5 min, displayed maximum activity at 37°C. Treatment with higher temperatures caused inactivation of enzyme activity. The enzyme followed a simple Michaelis-Menten curve when assayed at varying substrate concentration and yielded Km value to be 0.0370 mM. When a fixed activity of AChE was assayed in presence of different concentrations of cadmium, the enzyme activity was sharply reduced; the IC50 value being about 5.7mM. The enzyme when assayed in presence of cadmium at a concentration equal to its IC50, lost its 50% activity in 77 min (t1/2). Cadmium was found to act as a noncompetitive inhibitor to the enzyme. These results suggested that AChE from rat brain may serve as a significant biomarker of cadmium induced neurotoxicity..

https://juniperpublishers.com/oajt/pdf/OAJT.MS.ID.555553.pdf

https://juniperpublishers.com/oajt/OAJT.MS.ID.555553.php