Tuesday, November 27, 2018

Nongenomic Effect of T3 on the Na, K-ATPase Activity of Syncytiotrophoblast from Human Term Placenta

Authored by Fulgencio Proverbio

Several ion transport enzymes are positively regulated by genomic mechanisms, through the thyroid hormones T3 and T4. However, there is evidence indicating that these hormones might also exert non genomic effects on different transporting enzymes. A normal activity of the Na, K-ATPase of  syncytiotrophoblast is essential for ion homeostasis in this tissue, as well as for the growth and development of the fetus. In the current work it was studied the non genomic effect of thyroid hormones on the Na, K-ATPase activity of syncytiotrophoblast. The study was carried-out by incubating human term placental explants in the absence and presence of 10nMT3or 350nMT4 and then isolating the syncytiotrophoblast plasma membranes and assaying them for Na, K-ATPase activity. The enzyme activity was also assayed for freshly prepared plasma membranes in the presence of (0-15)nMT3 or (0-500)nMT4.

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