Regenerative medicine requires adequate physical
supports to facilitate appropriate cellular architecture, cell
polarization and guidance and the improvement of the correct
differentiation processes of embryonic or adult stem cells and induced
pluripotent cells. Interdisciplinary collaboration between cell
biologists, physicists and engineers is required to develop and
successfully achieve the objectives of this pioneering research in
tissue engineering. We try, in these lines, to highlight some of the
technical and methodological issues useful in the research of
graphene-based nanomaterial cytocompatibility, focused on its use in
biomedicine. The general goal must be increasing standardization,
precision and reproducibility of cellular assays. We observed a direct relationship between the
thickness and roughness of the graphene-based nanomaterials films and
cell maturation. Therefore, the importance to find the most suitable
thickness for inducing cell orientation, cell adherence and cell
maturation. Because these types of nanomaterials are synthesized under
highly controlled parameters, they might prove to be a powerful and
reproducible platform for use in biomedical applications, such as tissue
engineering and development of biocompatible devices.
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